Wiseman Justin M, Kennedy Joseph H
Prosolia, Inc., 351 West 10th Street, Indianapolis, IN, 46202, USA,
Methods Mol Biol. 2014;1198:291-7. doi: 10.1007/978-1-4939-1258-2_19.
Dried blood spot (DBS) analysis using mass spectrometry is an invaluable technique for examining blood markers of inborn metabolic diseases in clinical laboratories. Implementation of DBS sampling and analysis in pharmaceutical development have more recently gained traction due to the advantages of convenience in sample procurement and logistics, as well as the innate advantages associated with the collection of lower blood volumes. While there are several realized advantages of DBS, the bioanalytical laboratory is disadvantaged and burdened with additional preparative steps prior to analysis. Therefore, improvements in the laboratory workflow for DBS analysis are necessary. Here, we describe direct blood spot analysis using desorption electrospray ionization (DESI) mass spectrometry for quantitative determination of drugs in whole blood.
使用质谱法进行干血斑（DBS）分析是临床实验室检测先天性代谢疾病血液标志物的一项重要技术。由于在样本采集和物流方面具有便利性优势，以及与采集较少血量相关的固有优势，近年来，DBS采样和分析在药物研发中的应用越来越受到关注。虽然DBS有几个已实现的优势，但生物分析实验室在分析前处于劣势，并且负担着额外的制备步骤。因此，有必要改进DBS分析的实验室工作流程。在此，我们描述了使用解吸电喷雾电离（DESI）质谱法对全血中的药物进行定量测定的直接血斑分析。
