Payne Matthew S, Feng Zhenhua, Li Shaofu, Doherty Dorota A, Xu Biyun, Li Jie, Liu Lenan, Keelan Jeffrey A, Zhou Yi Hua, Dickinson Jan E, Hu Yali, Newnham John P
School of Women's and Infants' Health, The University of Western Australia, King Edward Memorial Hospital, 374 Bagot Road, Subiaco 6008, Western Australia, Australia.
BMC Pregnancy Childbirth. 2014 Sep 30;14:340. doi: 10.1186/1471-2393-14-340.
This study tested if second trimester amniotic fluid cytokine levels, Ureaplasma sp. colonisation and sexual activity predict preterm birth and explain the differential preterm birth rates in Chinese compared to Australian women.
Amniotic fluid was collected by amniocentesis (Chinese 480, Australian 492). Cytokines were measured by multiplex assay and Ureaplasma sp. DNA was detected by PCR analysis. Lifestyle factors, including history of smoking and sexual activity during pregnancy, were obtained through completion of questionnaires upon recruitment to the study.
Inflammatory cytokine concentrations were poorly predictive of preterm birth. Ureaplasma sp. was detected in two of the Chinese pregnancies and none from Australia. Sexual activity was less frequent in Chinese, and was not associated with preterm birth or amniotic fluid findings in either population.
Second trimester amniocentesis for measurement of inflammatory markers and Ureaplasma sp. DNA was not indicative of risk of preterm birth, at least in these populations. The lower rate of preterm birth in China was not explained by differences in amniotic fluid inflammatory markers, Ureaplasma sp. colonisation, or sexual activity.
本研究旨在检验孕中期羊水细胞因子水平、解脲脲原体定植情况及性行为是否可预测早产，并解释中国女性与澳大利亚女性早产率差异的原因。
通过羊膜穿刺术收集羊水（中国480例，澳大利亚492例）。采用多重检测法测定细胞因子，通过聚合酶链反应分析检测解脲脲原体DNA。生活方式因素，包括吸烟史和孕期性行为，通过研究招募时填写问卷获得。
炎症细胞因子浓度对早产的预测性较差。在中国的两例妊娠中检测到了解脲脲原体，而澳大利亚的妊娠中均未检测到。中国女性的性行为频率较低，且在任何人群中，性行为均与早产或羊水检查结果无关。
至少在这些人群中，孕中期羊膜穿刺术检测炎症标志物和解脲脲原体DNA并不能提示早产风险。中国较低的早产率无法通过羊水炎症标志物、解脲脲原体定植或性行为的差异来解释。
