Zhou Zhengzheng, Luo Jianguang, Wang Junsong, Li Liang, Kong Lingyi
a Department of Natural Medicinal Chemistry , China Pharmaceutical University , 24 Tong Jia Xiang, Nanjing 210009 , P.R. China.
Nat Prod Res. 2015;29(2):185-8. doi: 10.1080/14786419.2014.964704. Epub 2014 Oct 3.
An efficient, feasible enrichment and separation method of epimedins A, B, C and icariin from Herba Epimedii was developed by the combination of microwave-assisted extraction, macroporous resins and preparative HPLC. WDX-5 macroporous resin shows better recoveries at 96.2%, 97.0%, 98.2% and 97.1% for epimedins A, B, C and icariin than other macroporous resins used in the experiments. As a result, epimedins A (5.1 mg), B (15.3 mg), C (7.6 mg) and icariin (14.3 mg) were obtained from 6.0 g crude Herba Epimedii with the recoveries at 70.8%, 68.9%, 66.7% and 95.3%, respectively. The method developed in this study may provide scientific references for the enrichment and separation of flavonoids from Herba Epimedii.
通过微波辅助萃取、大孔树脂和制备型高效液相色谱相结合的方法，开发了一种从淫羊藿中高效、可行地富集和分离淫羊藿苷A、B、C及淫羊藿苷的方法。WDX - 5大孔树脂对淫羊藿苷A、B、C及淫羊藿苷的回收率分别为96.2%、97.0%、98.2%和97.1%，优于实验中使用的其他大孔树脂。结果，从6.0 g淫羊藿粗品中获得了淫羊藿苷A（5.1 mg）、B（15.3 mg）、C（7.6 mg）和淫羊藿苷（14.3 mg），回收率分别为70.8%、68.9%、66.7%和95.3%。本研究开发的方法可为从淫羊藿中富集和分离黄酮类化合物提供科学参考。
