Guo Yani, Wang Xiufeng, Gao Jing
School of Materials Science & Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, P. R. China.
Center for Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, P. R. China.
Chem Biodivers. 2018 Apr;15(4):e1700578. doi: 10.1002/cbdv.201700578. Epub 2018 Apr 10.
In this study, epimedins A-, B-, and C-, and icariin-rich extracts were simultaneously isolated from Epimedium brevicornu Maxim. through a convenient four-stage process consisting of solvent extraction, macroporous resin column pre-concentration, extraction fractionation, and reversed-phase (RP) silica gel column chromatography. Single factor experiments and response surface methodology (RSM) were used to optimize the preparation conditions. In the final products, the purities of epimedins A - C and icariin were 23.04%, 64.50%, 54.92%, and 77.54%, respectively, which will lay a foundation for the further purification of epimedin monomers and full utilization of Epimedium resources. Meanwhile, the osteogenic effects of epimedins A - C were investigated for the first time and compared with that of icariin, which will provide guidance for the clinical applications of Epimedium in the treatment of osteoporosis.
在本研究中，通过由溶剂萃取、大孔树脂柱预浓缩、萃取分级和反相（RP）硅胶柱色谱组成的便捷四步工艺，同时从短角淫羊藿中分离出富含淫羊藿苷A、B、C和淫羊藿苷的提取物。采用单因素实验和响应面法（RSM）优化制备条件。最终产物中，淫羊藿苷A - C和淫羊藿苷的纯度分别为23.04%、64.50%、54.92%和77.54%，这将为淫羊藿苷单体的进一步纯化和淫羊藿资源的充分利用奠定基础。同时，首次研究了淫羊藿苷A - C的成骨作用，并与淫羊藿苷进行了比较，这将为淫羊藿在骨质疏松症治疗中的临床应用提供指导。
