利用荧光显微镜图像捕获技术对大鼠膝关节进行三维重建。
Three-dimensional reconstruction of rat knee joint using episcopic fluorescence image capture.
机构信息
Human Health Science, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Human Health Science, Graduate School of Medicine, Kyoto University, Kyoto, Japan; Congenital Anomaly Research Center, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
出版信息
Osteoarthritis Cartilage. 2014 Oct;22(10):1401-9. doi: 10.1016/j.joca.2014.06.016.
OBJECTIVE
Development of the knee joint was morphologically investigated, and the process of cavitation was analyzed by using episcopic fluorescence image capture (EFIC) to create spatial and temporal three-dimensional (3D) reconstructions.
METHODS
Knee joints of Wister rat embryos between embryonic day (E)14 and E20 were investigated. Samples were sectioned and visualized using an EFIC. Then, two-dimensional image stacks were reconstructed using OsiriX software, and 3D reconstructions were generated using Amira software.
RESULTS
Cavitations of the knee joint were constructed from five divided portions. Cavity formation initiated at multiple sites at E17; among them, the femoropatellar cavity (FPC) was the first. Cavitations of the medial side preceded those of the lateral side. Each cavity connected at E20 when cavitations around the anterior cruciate ligament (ACL) and posterior cruciate ligament (PCL) were completed.
CONCLUSION
Cavity formation initiated from six portions. In each portion, development proceeded asymmetrically. These results concerning anatomical development of the knee joint using EFIC contribute to a better understanding of the structural feature of the knee joint.
目的
通过使用荧光透视图像捕获（EFIC）进行形态学研究，分析膝关节的空化过程，以创建时空三维（3D）重建。
方法
研究了 E14 至 E20 天 Wistar 大鼠胚胎的膝关节。使用 EFIC 对样本进行切片和可视化。然后，使用 OsiriX 软件重建二维图像堆栈，并使用 Amira 软件生成 3D 重建。
结果
膝关节的空化从五个分开的部分构建。在 E17 时，多个部位开始形成空洞；其中，髌股关节腔（FPC）是第一个。内侧的空化先于外侧的空化。当前交叉韧带（ACL）和后交叉韧带（PCL）周围的空化完成时，每个腔在 E20 时连接。
结论
从六个部分开始形成腔。在每个部分中，发育都是不对称进行的。这些使用 EFIC 对膝关节解剖发育的研究结果有助于更好地理解膝关节的结构特征。
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