Vijayakumar S, Vinoj G, Malaikozhundan B, Shanthi S, Vaseeharan B
Crustacean Molecular Biology and Genomics Lab, Department of Animal Health and Management, Alagappa University, Karaikudi 630004, Tamil Nadu, India.
Crustacean Molecular Biology and Genomics Lab, Department of Animal Health and Management, Alagappa University, Karaikudi 630004, Tamil Nadu, India.
Spectrochim Acta A Mol Biomol Spectrosc. 2015 Feb 25;137:886-91. doi: 10.1016/j.saa.2014.08.064. Epub 2014 Sep 16.
In this study, zinc oxide nanoparticles were biologically synthesized using the leaf extract of Plectranthus amboinicus (Pam-ZnO NPs). The synthesized Pam-ZnO NPs were characterized by UV-Vis spectrophotometer, FTIR, TEM and XRD analysis. TEM analysis of Pam-ZnO NPs showed the average size of about 20-50 nm. Pam-ZnO NPs control the growth of methicillin-resistant Staphylococcus aureus biofilms (MRSA ATCC 33591) at the concentration of 8-10 μg/ml. Confocal laser scanning microscope (CLSM) images revealed that Pam-ZnO NPs strongly inhibited the biofilm forming ability of S. aureus. In addition, Pam-ZnO NPs showed 100% mortality of fourth instar mosquito larvae of Anopheles stephensi, Culex quinquefasciatus and Culex tritaeniorhynchus at the concentration of 8 and 10 μg/ml. The histopathological studies of Pam-ZnO NPs treated A. stephensi and C. quinquefasciatus larvae revealed the presence of damaged cells and tissues in the mid-gut. The damaged tissues suffered major changes including rupture and disintegration of epithelial layer and cellular vacuolization. The present study conclude that Pam-ZnO NPs showed effective control of S. aureus biofilms and mosquito larvae by damaging the mid gut cells.
在本研究中，利用广藿香叶提取物生物合成了氧化锌纳米颗粒（Pam-ZnO NPs）。通过紫外可见分光光度计、傅里叶变换红外光谱仪、透射电子显微镜和X射线衍射分析对合成的Pam-ZnO NPs进行了表征。Pam-ZnO NPs的透射电子显微镜分析显示其平均尺寸约为20-50纳米。Pam-ZnO NPs在浓度为8-10微克/毫升时可控制耐甲氧西林金黄色葡萄球菌生物膜（MRSA ATCC 33591）的生长。共聚焦激光扫描显微镜（CLSM）图像显示，Pam-ZnO NPs强烈抑制金黄色葡萄球菌的生物膜形成能力。此外，Pam-ZnO NPs在浓度为8和10微克/毫升时对斯氏按蚊、致倦库蚊和三带喙库蚊的四龄幼虫显示出100%的死亡率。对Pam-ZnO NPs处理的斯氏按蚊和致倦库蚊幼虫的组织病理学研究显示，中肠存在受损的细胞和组织。受损组织发生了重大变化，包括上皮层破裂和崩解以及细胞空泡化。本研究得出结论，Pam-ZnO NPs通过损伤中肠细胞对金黄色葡萄球菌生物膜和蚊虫幼虫显示出有效的控制作用。
