da Silva Junielson Soares, da Silva Pinto Ana Cristina, de Souza Samara Silva, Chaves Francisco Célio Maia, da Fonseca Meireles Sabrina, Pereira Rosalina Pinheiro, Roque Rosemary Aparecida, de Castro E Sousa João Marcelo, Rafael Míriam Silva
Postgraduate Program in Genetics, Conservation and Evolutionary Biology, Coordination of Society, Environment and Health, National Institute of Amazonian Research, Manaus 69060-001, AM, Brazil.
Laboratory of Malaria and Dengue, Coordination of Society, Environment and Health, National Institute of Amazonian Research, Manaus 69067-375, AM, Brazil.
Toxics. 2025 Apr 8;13(4):283. doi: 10.3390/toxics13040283.
The study of substances of botanical origin is fundamental for the development of new effective alternatives for the control of (Culicidae), a vector of arboviruses in humans. In this study, the potential of two new dillapiole derivatives, propyl ether dillapiole and piperidyl dillapiole, was tested to determine their ability to deter oviposition and their larvicidal and residual effects against under simulated field conditions, as alternatives for the control of this mosquito. The ability of these substances to deter oviposition by pregnant females was assessed in the laboratory, and then the larvicide and residual effects of different concentrations were tested under simulated field conditions. The determination of the enzymatic activity in exposed larvae was carried out using sublethal concentrations. The LC values of propyl ether dillapiole after 24 and 48 h were 24.60 µg/mL and 14.76 µg/mL, and those of piperidyl dillapiole were 31.58 µg/mL and 24.85 µg/mL, respectively. After 48 h of exposure to aged, treated water, the mortality of propyl ether dillapiole (100 µg/mL) and piperidyl dillapiole (200 µg/mL) fell to 81.7% and 75% on the second day, and to 73.3% and 66.7% on the fourth day, respectively. The concentrations of 100 µg/mL of propyl ether dillapiole and 200 µg/mL of piperidyl dillapiole caused oviposition rates of only 3.80% and 4.63% of the eggs of the females, respectively, compared to 22.01% in the negative control (water and DMSO at 2%). In the larvae exposed to propyl ether, piperidyl dillapiole, dillapiole, or the chemical insecticide temephos (positive control), inhibition of acetylcholinesterase (AChE) occurred. Propyl ether dillapiole and piperidyl dillapiole have potential for use as alternative forms of control of , with propyl ether dillapiole being the most promising molecule. Further studies are needed to understand the effects of these substances on this mosquito and on non-target organisms.
对植物源物质的研究是开发控制人类虫媒病毒载体库蚊（蚊科）新的有效替代方法的基础。在本研究中，测试了两种新的莳萝脑衍生物——丙醚莳萝脑和哌啶基莳萝脑的潜力，以确定它们在模拟田间条件下阻止产卵的能力以及对库蚊的杀幼虫和残留效果，作为控制这种蚊子的替代方法。在实验室中评估了这些物质阻止怀孕库蚊雌蚊产卵的能力，然后在模拟田间条件下测试了不同浓度的杀幼虫剂和残留效果。使用亚致死浓度测定暴露幼虫中的酶活性。丙醚莳萝脑在24小时和48小时后的LC值分别为24.60μg/mL和14.76μg/mL，哌啶基莳萝脑的LC值分别为31.58μg/mL和24.85μg/mL。在暴露于老化的处理过的水48小时后，丙醚莳萝脑（100μg/mL）和哌啶基莳萝脑（200μg/mL）的死亡率在第二天分别降至81.7%和75%，在第四天分别降至73.3%和66.7%。与阴性对照（水和2%的二甲基亚砜）中的22.01%相比，100μg/mL的丙醚莳萝脑和200μg/mL的哌啶基莳萝脑浓度分别仅导致雌蚊产卵率为3.80%和4.63%。在暴露于丙醚、哌啶基莳萝脑、莳萝脑或化学杀虫剂双硫磷（阳性对照）的幼虫中，发生了乙酰胆碱酯酶（AChE）的抑制。丙醚莳萝脑和哌啶基莳萝脑有潜力用作控制库蚊的替代形式，其中丙醚莳萝脑是最有前景的分子。需要进一步研究以了解这些物质对这种蚊子和非靶标生物的影响。
