Bioprocess Engineering, AlgaePARC, Wageningen University, PO Box 16, 6700 AA Wageningen, The Netherlands.
Bioprocess Engineering, AlgaePARC, Wageningen University, PO Box 16, 6700 AA Wageningen, The Netherlands.
Bioresour Technol. 2015 May;184:297-304. doi: 10.1016/j.biortech.2014.09.033. Epub 2014 Sep 16.
In this work, the mild disintegration of the microalgae Chlorella vulgaris for the release of intracellular products has been studied. By means of bead milling the microalgae suspensions were successfully disintegrated at different biomass concentrations (25-145 gDW kg(-1)) over a range of agitator speeds (6-12 m s(-1)). In all cases over 97% of cell disintegration was achieved resulting in a release of water soluble proteins. A clear optimum rate of disintegration and protein release was observed at an agitator speed of 9-10 m s(-1) regardless of the biomass concentration. Selective extraction of water soluble proteins was observed as proteins released sooner than cell disintegration took place. Proteins could be released at 85% lower energy input than for cell disintegration resulting in specific energy consumptions well below 2.5 kWh kgDW(-1).
本工作研究了小球藻的温和细胞破碎以释放胞内产物。通过珠磨，在不同的生物质浓度（25-145 gDW kg(-1))和搅拌速度（6-12 m s(-1))范围内，成功地破碎了微藻悬浮液。在所有情况下，细胞破碎率均超过 97%，导致水溶性蛋白质释放。无论生物质浓度如何，在搅拌速度为 9-10 m s(-1)时，均观察到明显的最佳破碎率和蛋白质释放率。由于细胞破碎发生之前，蛋白质就已经释放出来，因此观察到了水溶性蛋白质的选择性提取。与细胞破碎相比，蛋白质释放所需的能量输入低 85%，导致比消耗能低于 2.5 kWh kgDW(-1)。
