具有短天然外显肽和活性N端催化半胱氨酸的小型RecA内含肽的主链归属
Backbone assignments of mini-RecA intein with short native exteins and an active N-terminal catalytic cysteine.
作者信息
Pearson C Seth, Belfort Georges, Belfort Marlene, Shekhtman Alexander
机构信息
Howard P. Isermann Department of Chemical and Biological Engineering and The Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.
Department of Biological Sciences and The RNA Institute, University at Albany, Albany, NY, 12222, USA.
出版信息
Biomol NMR Assign. 2015 Oct;9(2):235-8. doi: 10.1007/s12104-014-9581-z. Epub 2014 Oct 4.
Abstract
The backbone resonance assignments of an engineered splicing-inactive mini-RecA intein based on triple resonance experiments with [(13)C,(15)N]-labeled protein are reported. The construct contains inactivating mutations specifically designed to retain most catalytic residues, especially those that are potentially metal-coordinating. The assignments are essential for protein structure determination of a precursor with an active N-terminal catalytic cysteine and for investigation of the atomic details of splicing.
摘要
报道了基于对[(13)C,(15)N]标记蛋白质进行三重共振实验的工程化剪接失活型迷你RecA内含肽的主链共振归属。该构建体包含专门设计的失活突变，以保留大多数催化残基，特别是那些可能参与金属配位的残基。这些归属对于具有活性N端催化半胱氨酸的前体的蛋白质结构测定以及剪接原子细节的研究至关重要。
相似文献
1
Backbone assignments of mini-RecA intein with short native exteins and an active N-terminal catalytic cysteine.具有短天然外显肽和活性N端催化半胱氨酸的小型RecA内含肽的主链归属
Biomol NMR Assign. 2015 Oct;9(2):235-8. doi: 10.1007/s12104-014-9581-z. Epub 2014 Oct 4.
2
Dynamics differentiate between active and inactive inteins.动力学可区分活性内含肽和非活性内含肽。
Eur J Med Chem. 2015 Feb 16;91:51-62. doi: 10.1016/j.ejmech.2014.07.094. Epub 2014 Jul 27.
3
1H, 13C, and 15N NMR assignments of an engineered intein based on Mycobacterium tuberculosis RecA.基于结核分枝杆菌RecA的工程化内含肽的1H、13C和15N核磁共振归属
Biomol NMR Assign. 2008 Dec;2(2):111-3. doi: 10.1007/s12104-008-9098-4. Epub 2008 May 30.
4
Methods to Study the Structure and Catalytic Activity of cis-Splicing Inteins.研究顺式剪接内含子结构和催化活性的方法。
Methods Mol Biol. 2020;2133:55-73. doi: 10.1007/978-1-0716-0434-2_4.
5
Post-translational environmental switch of RadA activity by extein-intein interactions in protein splicing.蛋白质剪接过程中通过外显肽-内含肽相互作用实现的RadA活性的翻译后环境开关。
Nucleic Acids Res. 2015 Jul 27;43(13):6631-48. doi: 10.1093/nar/gkv612. Epub 2015 Jun 22.
6
Minimization and stabilization of the Mycobacterium tuberculosis recA intein.结核分枝杆菌recA内含肽的最小化与稳定化
J Mol Biol. 2005 Dec 9;354(4):916-26. doi: 10.1016/j.jmb.2005.09.088. Epub 2005 Oct 21.
7
Branched intermediate formation is the slowest step in the protein splicing reaction of the Ala1 KlbA intein from Methanococcus jannaschii.分支中间体的形成是产甲烷球菌 Ala1 KlbA 内含肽蛋白剪接反应中最慢的步骤。
Biochemistry. 2011 Dec 13;50(49):10576-89. doi: 10.1021/bi200810j. Epub 2011 Nov 16.
8
Reactivity of the cysteine residues in the protein splicing active center of the Mycobacterium tuberculosis RecA intein.结核分枝杆菌RecA内含肽蛋白剪接活性中心中半胱氨酸残基的反应性
Arch Biochem Biophys. 2000 Mar 1;375(1):138-44. doi: 10.1006/abbi.1999.1645.
9
Conditional protein splicing of the Mycobacterium tuberculosis RecA intein in its native host.结核分枝杆菌 RecA 内含肽在其天然宿主中的条件性蛋白剪接。
Sci Rep. 2024 Sep 5;14(1):20664. doi: 10.1038/s41598-024-71248-y.
10
Backbone dynamics and global effects of an activating mutation in minimized Mtu RecA inteins.最小化 Mtu RecA 内含肽中的激活突变对骨架动力学和全局的影响。
J Mol Biol. 2010 Jul 23;400(4):755-67. doi: 10.1016/j.jmb.2010.05.044. Epub 2010 May 24.
引用本文的文献
1
Exploring Intein Inhibition by Platinum Compounds as an Antimicrobial Strategy.探索铂化合物抑制内含肽作为一种抗菌策略。
J Biol Chem. 2016 Oct 21;291(43):22661-22670. doi: 10.1074/jbc.M116.747824. Epub 2016 Sep 8.
本文引用的文献
1
Structure of the branched intermediate in protein splicing.蛋白质剪接中分支中间体的结构。
Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):8422-7. doi: 10.1073/pnas.1402942111. Epub 2014 Apr 28.
2
Recent advances in in vivo applications of intein-mediated protein splicing.体内应用内含肽介导的蛋白质剪接的最新进展。
Mob DNA. 2014 Feb 4;5(1):5. doi: 10.1186/1759-8753-5-5.
3
A conserved threonine spring-loads precursor for intein splicing.一个保守的苏氨酸弹簧装载前体用于内含子剪接。
Protein Sci. 2013 May;22(5):557-63. doi: 10.1002/pro.2236. Epub 2013 Mar 26.
4
NMR and crystal structures of the Pyrococcus horikoshii RadA intein guide a strategy for engineering a highly efficient and promiscuous intein.Pyrococcus horikoshii RadA 内含肽的 NMR 和晶体结构为设计高效广谱内含肽提供了策略。
J Mol Biol. 2012 Aug 3;421(1):85-99. doi: 10.1016/j.jmb.2012.04.029. Epub 2012 May 2.
5
Structural and mutational studies of a hyperthermophilic intein from DNA polymerase II of Pyrococcus abyssi.嗜热古菌 Pyrococcus abyssi DNA 聚合酶 II 内含肽的结构和突变研究。
J Biol Chem. 2011 Nov 4;286(44):38638-38648. doi: 10.1074/jbc.M111.290569. Epub 2011 Sep 13.
6
Directed evolution of a small-molecule-triggered intein with improved splicing properties in mammalian cells.在哺乳动物细胞中具有改进剪接特性的小分子触发内含肽的定向进化。
Chem Biol. 2011 May 27;18(5):619-30. doi: 10.1016/j.chembiol.2011.02.014.
7
pK(a) coupling at the intein active site: implications for the coordination mechanism of protein splicing with a conserved aspartate.内含肽活性位点的 pK(a) 偶联：对保守天冬氨酸参与蛋白质剪接的协调机制的启示。
J Am Chem Soc. 2011 Jul 6;133(26):10275-82. doi: 10.1021/ja203209f. Epub 2011 Jun 9.
8
Structure of catalytically competent intein caught in a redox trap with functional and evolutionary implications.具有催化活性的内含肽在氧化还原陷阱中的结构，具有功能和进化意义。
Nat Struct Mol Biol. 2011 May;18(5):630-3. doi: 10.1038/nsmb.2041. Epub 2011 Apr 3.
9
Spontaneous proton transfer to a conserved intein residue determines on-pathway protein splicing.自发质子转移到保守的内含肽残基决定了蛋白内含肽的途径蛋白剪接。
J Mol Biol. 2011 Feb 25;406(3):430-42. doi: 10.1016/j.jmb.2010.12.024. Epub 2010 Dec 23.
10
Cisplatin inhibits protein splicing, suggesting inteins as therapeutic targets in mycobacteria.顺铂抑制蛋白质剪接，提示整合酶作为分枝杆菌的治疗靶点。
J Biol Chem. 2011 Jan 14;286(2):1277-82. doi: 10.1074/jbc.M110.171124. Epub 2010 Nov 8.
Zotero 插件