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2
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本文引用的文献

1
Structure of the branched intermediate in protein splicing.蛋白质剪接中分支中间体的结构。
Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):8422-7. doi: 10.1073/pnas.1402942111. Epub 2014 Apr 28.
2
Recent advances in in vivo applications of intein-mediated protein splicing.体内应用内含肽介导的蛋白质剪接的最新进展。
Mob DNA. 2014 Feb 4;5(1):5. doi: 10.1186/1759-8753-5-5.
3
A conserved threonine spring-loads precursor for intein splicing.一个保守的苏氨酸弹簧装载前体用于内含子剪接。
Protein Sci. 2013 May;22(5):557-63. doi: 10.1002/pro.2236. Epub 2013 Mar 26.
4
NMR and crystal structures of the Pyrococcus horikoshii RadA intein guide a strategy for engineering a highly efficient and promiscuous intein.Pyrococcus horikoshii RadA 内含肽的 NMR 和晶体结构为设计高效广谱内含肽提供了策略。
J Mol Biol. 2012 Aug 3;421(1):85-99. doi: 10.1016/j.jmb.2012.04.029. Epub 2012 May 2.
5
Structural and mutational studies of a hyperthermophilic intein from DNA polymerase II of Pyrococcus abyssi.嗜热古菌 Pyrococcus abyssi DNA 聚合酶 II 内含肽的结构和突变研究。
J Biol Chem. 2011 Nov 4;286(44):38638-38648. doi: 10.1074/jbc.M111.290569. Epub 2011 Sep 13.
6
Directed evolution of a small-molecule-triggered intein with improved splicing properties in mammalian cells.在哺乳动物细胞中具有改进剪接特性的小分子触发内含肽的定向进化。
Chem Biol. 2011 May 27;18(5):619-30. doi: 10.1016/j.chembiol.2011.02.014.
7
pK(a) coupling at the intein active site: implications for the coordination mechanism of protein splicing with a conserved aspartate.内含肽活性位点的 pK(a) 偶联：对保守天冬氨酸参与蛋白质剪接的协调机制的启示。
J Am Chem Soc. 2011 Jul 6;133(26):10275-82. doi: 10.1021/ja203209f. Epub 2011 Jun 9.
8
Structure of catalytically competent intein caught in a redox trap with functional and evolutionary implications.具有催化活性的内含肽在氧化还原陷阱中的结构，具有功能和进化意义。
Nat Struct Mol Biol. 2011 May;18(5):630-3. doi: 10.1038/nsmb.2041. Epub 2011 Apr 3.
9
Spontaneous proton transfer to a conserved intein residue determines on-pathway protein splicing.自发质子转移到保守的内含肽残基决定了蛋白内含肽的途径蛋白剪接。
J Mol Biol. 2011 Feb 25;406(3):430-42. doi: 10.1016/j.jmb.2010.12.024. Epub 2010 Dec 23.
10
Cisplatin inhibits protein splicing, suggesting inteins as therapeutic targets in mycobacteria.顺铂抑制蛋白质剪接，提示整合酶作为分枝杆菌的治疗靶点。
J Biol Chem. 2011 Jan 14;286(2):1277-82. doi: 10.1074/jbc.M110.171124. Epub 2010 Nov 8.

具有短天然外显肽和活性N端催化半胱氨酸的小型RecA内含肽的主链归属

Backbone assignments of mini-RecA intein with short native exteins and an active N-terminal catalytic cysteine.

作者信息

Pearson C Seth, Belfort Georges, Belfort Marlene, Shekhtman Alexander

机构信息

Howard P. Isermann Department of Chemical and Biological Engineering and The Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.

Department of Biological Sciences and The RNA Institute, University at Albany, Albany, NY, 12222, USA.

出版信息

Biomol NMR Assign. 2015 Oct;9(2):235-8. doi: 10.1007/s12104-014-9581-z. Epub 2014 Oct 4.

DOI:10.1007/s12104-014-9581-z
PMID:25281002
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4385508/
Abstract

The backbone resonance assignments of an engineered splicing-inactive mini-RecA intein based on triple resonance experiments with [(13)C,(15)N]-labeled protein are reported. The construct contains inactivating mutations specifically designed to retain most catalytic residues, especially those that are potentially metal-coordinating. The assignments are essential for protein structure determination of a precursor with an active N-terminal catalytic cysteine and for investigation of the atomic details of splicing.

摘要

报道了基于对[(13)C,(15)N]标记蛋白质进行三重共振实验的工程化剪接失活型迷你RecA内含肽的主链共振归属。该构建体包含专门设计的失活突变，以保留大多数催化残基，特别是那些可能参与金属配位的残基。这些归属对于具有活性N端催化半胱氨酸的前体的蛋白质结构测定以及剪接原子细节的研究至关重要。