College of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou 225002, China; Yangzhou Polytechnic Institute, Yangzhou 225127, China.
College of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou 225002, China.
Talanta. 2015 Jan;131:603-8. doi: 10.1016/j.talanta.2014.08.021. Epub 2014 Aug 23.
In this paper, a simple and efficient ultrasound-assisted ionic liquid dispersive liquid-liquid microextraction (UA IL-DLLME) coupled with high-performance liquid chromatography for the analysis of ulipristal acetate (UPA) was developed. UPA could be easily migrated into 1-octyl-3-methylimidazolium hexafluorophosphate [C8mimPF6] IL phase without dispersive solvent. The research of extraction mechanism showed that hydrophobic interaction force played a key role in the IL-DLLME. Several important parameters affecting the extraction recovery were optimized. Under the optimized conditions, 25-fold enrichment factor was obtained and the limit of detection (LOD) was 6.8 ng mL(-1) (tablet) or 9.3 ng mL(-1) (serum) at a signal-to-noise ratio of 3. The calibration curve was linear over the range of 0.03-6.0 µg mL(-1). The proposed method was successfully applied to the UPA tablets and the real mice serum samples.
本文建立了一种简单高效的超声辅助离子液体分散液液微萃取（UA-IL-DLLME）与高效液相色谱相结合的方法，用于分析屈螺酮（UPA）。UPA 可无需分散溶剂而轻易迁移至 1-辛基-3-甲基咪唑六氟磷酸盐 [C8mimPF6] IL 相。萃取机制的研究表明，疏水力在 IL-DLLME 中起着关键作用。优化了几个影响萃取回收率的重要参数。在优化条件下，获得了 25 倍的富集因子，检测限（LOD）在信噪比为 3 时，片剂为 6.8 ng mL(-1)（片剂）或 9.3 ng mL(-1)（血清），校准曲线在 0.03-6.0 µg mL(-1)范围内呈线性。该方法成功应用于 UPA 片剂和真实小鼠血清样品的分析。
