Institute of Environmental Science, and School of Chemistry and Chemical Engineering, Shanxi University, 92 Wucheng Road, Taiyuan 030006, China; Biology Institute of Shanxi, 50 Shifan Street, Taiyuan 030006, China.
Institute of Environmental Science, and School of Chemistry and Chemical Engineering, Shanxi University, 92 Wucheng Road, Taiyuan 030006, China.
Talanta. 2015 Jan;131:632-9. doi: 10.1016/j.talanta.2014.08.032. Epub 2014 Aug 21.
Metal nanoparticles (NPs) have recently attracted considerable attention in many areas of research including bioscience, chemistry and material science. Regrettably, most current and past work usually focuses on studies of multi-component NPs mixture where there is a plethora of NPs species co-existing. This work highlights the merits of reverse-phase high-performance liquid chromatography (RP-HPLC) for disclosing the genuine properties of individual palladium nanoparticles (PdNPs) species present in an as-synthesized N,N'-dimethylformamide-stabilized PdNPs product (DMF-PdNPs) which might have been previously hidden or misinterpreted. DMF-PdNPs is successfully separated by RP-HPLC that smaller DMF-PdNPs are approximately eluted first and then follow by the large ones on a C18 column. The separation fractions are further collected and determined their chemical compositions by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The results unambiguously reveal that the as-synthesized DMF-PdNPs product is indeed a complex mixture of ultrasmall PdxNPs (x=10-20) stabilized with different numbers of DMF ligands. It is anticipated that the separated fractions afforded by RP-HPLC will offer more accurate determinations of the catalytic, electronic, optical and toxicological properties of metal NPs which might have been previously misinterpreted.
金属纳米粒子 (NPs) 最近在包括生物科学、化学和材料科学在内的许多研究领域引起了相当大的关注。遗憾的是，目前大多数和过去的工作通常侧重于多组分 NPs 混合物的研究，其中存在大量的 NPs 物种共存。这项工作强调了反相高效液相色谱 (RP-HPLC) 的优点，它可以揭示合成的 N,N'-二甲基甲酰胺稳定的钯纳米粒子 (DMF-PdNPs) 产品中单个钯纳米粒子 (PdNPs) 物种的真实性质，这些性质可能以前被隐藏或误解了。DMF-PdNPs 可以通过 RP-HPLC 成功分离，较小的 DMF-PdNPs 首先被洗脱，然后在 C18 柱上紧随其后较大的 DMF-PdNPs。分离的馏分进一步收集并通过基质辅助激光解吸/电离飞行时间质谱法确定其化学成分。结果明确表明，合成的 DMF-PdNPs 产品实际上是一种与不同数量的 DMF 配体稳定的超小 PdxNPs (x=10-20) 的复杂混合物。预计 RP-HPLC 提供的分离馏分将更准确地确定金属 NPs 的催化、电子、光学和毒理学性质，这些性质以前可能被误解了。
