Research Center of Environmental Science and Engineering, Shanxi University, Taiyuan 030006, PR China.
Biosens Bioelectron. 2011 Jul 15;26(11):4619-23. doi: 10.1016/j.bios.2011.04.057. Epub 2011 May 6.
An amperometric glucose biosensor based on an n-alkylamine-stabilized palladium nanoparticles (PdNPs)-glucose oxidase (GOx) modified glassy carbon (GC) electrode has been successfully fabricated. PdNPs were initially synthesized by a biphase mixture of water and toluene method using n-alkylamines (dodecylamine, C₁₂-NH₂ and octadecylamine, C₁₈-NH₂) as stabilizing ligands. The performance of the PdNPs-GOx/GC biosensor was studied by cyclic voltammetry. The optimum working potential for amperometric measurement of glucose in pH 7.0 phosphate buffer solution is -0.02 V (vs. Ag/AgCl). The analytical performance of the biosensor prepared from C₁₈-PdNPs-GOx is better than that of C₁₂-PdNPs-GOx. The C₁₈-PdNPs-GOx/GC biosensor exhibits a fast response time of ca. 3s, a detection limit of 3.0 μM (S/N=3) and a linear range of 3.0 μM-8.0 mM. The linear dependence of current density with glucose concentration is 70.8 μA cm⁻² mM⁻¹. The biosensor shows good stability, repeatability and reproducibility. It has been successfully applied to determine the glucose content in human blood serum samples.
基于 n- 烷基胺稳定的钯纳米粒子（PdNPs）-葡萄糖氧化酶（GOx）修饰玻碳（GC）电极的安培葡萄糖生物传感器已成功制备。PdNPs 最初通过水和甲苯的双相混合物法合成，使用 n- 烷基胺（十二胺，C12-NH2 和十八胺，C18-NH2）作为稳定配体。通过循环伏安法研究了 PdNPs-GOx/GC 生物传感器的性能。在 pH 7.0 磷酸盐缓冲溶液中安培测量葡萄糖的最佳工作电位为-0.02 V（相对于 Ag/AgCl）。由 C18-PdNPs-GOx 制备的生物传感器的分析性能优于 C12-PdNPs-GOx。C18-PdNPs-GOx/GC 生物传感器具有约 3s 的快速响应时间、3.0 μM（S/N=3）的检测限和 3.0 μM-8.0 mM 的线性范围。电流密度与葡萄糖浓度的线性关系为 70.8 μA cm⁻² mM⁻¹。该生物传感器具有良好的稳定性、重复性和重现性。它已成功应用于测定人血清样品中的葡萄糖含量。
