用于细胞内 pH 成像的荧光比率探针。

A profluorescent ratiometric probe for intracellular pH imaging.

机构信息

Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, 398 Ruoshui Road, Suzhou Industrial Park, Suzhou, Jiangsu 215123, PR China; University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, PR China; Institute of Chemistry, Chinese Academy of Sciences, 2 Zhongguancun North First Street, Beijing 100190, PR China.

出版信息

Talanta. 2015 Jan;131:666-71. doi: 10.1016/j.talanta.2014.08.039. Epub 2014 Aug 23.

DOI:10.1016/j.talanta.2014.08.039

PMID:25281157

Abstract

A ratiometric pH probe composed of a fluorescein moiety and an ionic near-infrared-emitting phosphorescent cyclometalated iridium(III) complex bis(6-(benzo[b]thien-2-yl)phenanthridinato)(4-(3-carboxypropyl)-4'-methyl-2,2'-bipyridine)iridium(III) was synthesized. With good cell permeability, the probe demonstrated a linear ratiometric response to the pH variation in the physiological range in HeLa cell assay.

摘要

合成了一种比率型 pH 探针，由荧光素部分和离子近红外发射磷光铱（III）配合物双（6-（苯并[b]噻吩-2-基）菲咯啉）（4-（3-羧丙基）-4'-甲基-2,2'-联吡啶）铱（III）组成。该探针具有良好的细胞通透性，在 HeLa 细胞测定中对生理范围内 pH 值的变化表现出线性比率响应。