Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, 33006 Oviedo, Spain.
Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, 33006 Oviedo, Spain.
Talanta. 2015 Jan;131:706-11. doi: 10.1016/j.talanta.2014.08.013. Epub 2014 Aug 27.
The use of the first flow-cell for magnetic assays with an integrated magnet is reported here. The flow injection analysis system (FIA) is used for biotin determination. The reaction scheme is based on a one step competitive assay between free biotin and biotin labeled with horseradish peroxidase (B-HRP). The mixture of magnetic beads modified with streptavidin (Strep-MB), biotin and B-HRP is left 15 min under stirring and then a washing step is performed. After that, 100 μL of the mixture is injected and after 30s 100 μL of 3,3',5,5'-Tetramethylbenzidine (TMB) is injected and the FIAgram is recorded applying a potential of -0.2V. The linear range obtained is from 0.01 to 1 nM of biotin and the sensitivity is 758 nA/nM. The modification and cleaning of the electrode are performed in an easy way due to the internal magnet of the flow cell.
本文报道了一种使用集成磁铁的第一流池进行磁分析的方法。该流动注射分析系统（FIA）用于生物素的测定。该反应方案基于游离生物素与辣根过氧化物酶标记的生物素（B-HRP）之间的一步竞争测定。将链霉亲和素修饰的磁性珠（Strep-MB）、生物素和 B-HRP 的混合物在搅拌下反应 15 分钟，然后进行洗涤步骤。之后，注入 100μL 混合物，30 秒后注入 100μL 3,3',5,5'-四甲基联苯胺（TMB），并施加-0.2V 的电势记录 FIA 图谱。得到的线性范围为 0.01 至 1nM 的生物素，灵敏度为 758nA/nM。由于流池的内部磁铁，电极的修饰和清洗非常方便。
