Scott D M, Pateman J A
J Cell Physiol. 1978 Apr;95(1):57-63. doi: 10.1002/jcp.1040950108.
The uptake of L-alanine into BHK21-C13 cells in culture has been studied. This amino acid appears to be transported essentially via a relatively low affinity, high capacity, sodium ion dependent transport system. Inhibition studies using other amino acids or their analogues provided information about the specificity of this system. This alanine transport system was shown to exhibit a broad substrate specificity and appeared to be capable of transporting most naturally occurring neutral alpha-amino acids. Kinetic studies of the inhibition of L-alanine uptake also indicated the presence of a second neutral amino acid transport system capable of transporting this amino acid. However, it is unlikely that this second uptake system contributes greatly to L-alanine uptake. Inhibition of the uptake of L-leucine indicated that this transport system has a similar specificity to the "L"-system initially described for Ehrlich ascites carcinoma cells.
对培养的BHK21 - C13细胞摄取L - 丙氨酸的过程进行了研究。这种氨基酸似乎主要通过一种亲和力相对较低、容量较大、依赖钠离子的转运系统进行转运。使用其他氨基酸或其类似物的抑制研究提供了有关该系统特异性的信息。结果表明，这种丙氨酸转运系统具有广泛的底物特异性，似乎能够转运大多数天然存在的中性α - 氨基酸。对L - 丙氨酸摄取抑制的动力学研究还表明存在另一种能够转运这种氨基酸的中性氨基酸转运系统。然而，这种第二种摄取系统对L - 丙氨酸摄取的贡献可能不大。对L - 亮氨酸摄取的抑制表明，该转运系统与最初描述的艾氏腹水癌细胞的“L”系统具有相似的特异性。
