Wang X, Zhao P, Luo Q, Yan X, Xu J, Chen J, Chen H
Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation, Taizhou University, Taizhou, China.
Plant Biol (Stuttg). 2015 May;17(3):660-6. doi: 10.1111/plb.12273. Epub 2014 Dec 5.
Plant metabolomics is essentially the comprehensive analysis of complex metabolites of plant extracts. Metabolic fingerprinting is an important part of plant metabolomics research. In this study, metabolic fingerprinting of different stages of the life history of the red alga Porphyra haitanensis was performed. The stages included conchocelis filaments, sporangial branchlets, conchosporangia, discharged conchospores and conchosporangial branchlets after conchospore discharge. Metabolite extracts were analysed with ultra-performance liquid chromatography coupled with electrospray ionisation quadrupole-time of flight mass spectrometry. Analyses profiles were subjected to principal components analysis and orthogonal projection to latent structures discriminant analysis using the SIMCA-P software for biomarker selection and identification. Based on the MS/MS spectra and data from the literature, potential biomarkers, mainly of phosphatidylcholine and lysophosphatidylcholine, were identified. Identification of these biomarkers suggested that plasma membrane phospholipids underwent major changes during the life history of P. haitanensis. The levels of phosphatidylcholine and lysophosphatidylcholine increased in sporangial branchlets and decreased in discharged conchospores. Moreover, levels of sphingaine (d18:0) decreased in sporangial branchlets and increased in discharged conchospores, which indicates that membrane lipids were increasingly synthesised as energy storage in sporangial branchlets, while energy was consumed in sporangial branchlets to discharged conchospores. A metabolomic study of different growth phases of P. haitanensis will enhance our understanding of its physiology and ecology.
植物代谢组学本质上是对植物提取物中复杂代谢物的综合分析。代谢指纹分析是植物代谢组学研究的重要组成部分。在本研究中，对坛紫菜生活史不同阶段进行了代谢指纹分析。这些阶段包括壳孢子丝状体、孢子囊小枝、壳孢子囊、释放后的壳孢子以及壳孢子释放后的壳孢子囊小枝。代谢物提取物采用超高效液相色谱与电喷雾电离四极杆飞行时间质谱联用进行分析。分析图谱使用SIMCA-P软件进行主成分分析和正交投影到潜在结构判别分析，以选择和鉴定生物标志物。基于二级质谱图和文献数据，鉴定出了主要为磷脂酰胆碱和溶血磷脂酰胆碱的潜在生物标志物。这些生物标志物的鉴定表明，在坛紫菜的生活史中，质膜磷脂发生了重大变化。磷脂酰胆碱和溶血磷脂酰胆碱的水平在孢子囊小枝中升高，在释放后的壳孢子中降低。此外，鞘氨醇（d18:0）的水平在孢子囊小枝中降低，在释放后的壳孢子中升高，这表明膜脂在孢子囊小枝中作为能量储存而增加合成，而在从孢子囊小枝到释放后的壳孢子的过程中能量被消耗。对坛紫菜不同生长阶段的代谢组学研究将增进我们对其生理学和生态学的理解。
