Lin Yinghui, Xu Kai, Xu Yan, Ji Dehua, Chen Changsheng, Wang Wenlei, Xie Chaotian
Fisheries College, Jimei University, Xiamen, China.
Fujian Engineering Research Center of Aquatic Breeding and Healthy Aquaculture, Xiamen, China.
Front Genet. 2021 Jun 30;12:680120. doi: 10.3389/fgene.2021.680120. eCollection 2021.
Conchosporangia maturation is crucial for the yield of . However, the molecular mechanisms underlying this process are poorly understood. In this study, we selected two strains of that show significant differences in conchosporangia maturation as materials to produce RNA-Seq libraries. Then, we identified key molecular pathways and genes involved in conchosporangia maturation by conducting a weighted gene co-expression network analysis. Two specific modules were identified, and included functions such as phosphorus metabolism, lipid metabolism, and the phosphatidylinositol signaling system. The hub genes that responded positively during conchosporangia maturation encoded diacylglycerol kinase (DGK) and phosphatidylinositol-3-phosphate-5-kinase, which are involved in the synthesis of phosphatidic acid, a key component of lipid metabolism. A full-length sequence of , designated as , was obtained by rapid-amplification of cDNA ends. Conserved motif and phylogenetic tree analyses showed that belongs to DGK Cluster II. The transcript level of increased during conchosporangia maturation in both strains, but increased earlier, and to higher levels, in the early-maturing strain than in the late-maturing strain. This pattern of gene expression was consistent with the patterns of maturity and changes in pigment contents. These results indicate that lipid metabolism plays a key role in regulating conchosporangia maturation in spp., and that might be a useful molecular marker for breeding new early-maturing strains.
壳孢子囊成熟对[具体物种]的产量至关重要。然而，这一过程背后的分子机制却知之甚少。在本研究中，我们选择了两株在壳孢子囊成熟方面表现出显著差异的[具体物种]菌株作为材料来构建RNA测序文库。然后，通过进行加权基因共表达网络分析，我们鉴定出了参与壳孢子囊成熟的关键分子途径和基因。确定了两个特定模块，包括磷代谢、脂质代谢和磷脂酰肌醇信号系统等功能。在壳孢子囊成熟过程中呈阳性反应的枢纽基因编码二酰基甘油激酶（DGK）和磷脂酰肌醇-3-磷酸-5-激酶，它们参与脂质代谢关键成分磷脂酸的合成。通过cDNA末端快速扩增获得了一个[具体物种]的全长[基因名称]序列，命名为[具体名称]。保守基序和系统发育树分析表明[具体名称]属于DGK第二簇。在两株菌株的壳孢子囊成熟过程中，[具体名称]的转录水平均升高，但在早熟菌株中比晚熟菌株升高得更早且水平更高。这种基因表达模式与成熟模式和色素含量变化一致。这些结果表明脂质代谢在调控[具体物种]的壳孢子囊成熟中起关键作用，并且[具体名称]可能是培育新的早熟菌株的有用分子标记。
