Simard Carl, Cloutier Marc, Néron Sonia
Cellular Production, Research and Development, Héma-Québec, Canada.
J Immunol Methods. 2014 Dec 15;415:63-5. doi: 10.1016/j.jim.2014.09.005. Epub 2014 Oct 5.
Current methods to measure the specific activity of cytokines are based on the time-consuming determination of the growth curve of a sensitive cell line. Here, we present a faster alternative based on flow cytometry, by determining the dose-response curve of cellular response to a cytokine. By using World Health Organization (WHO) cytokine standards, rapid determination of cytokine specific activity is now possible, as it takes only a few hours to achieve, in comparison to days with the classical method thus allowing laboratories to rapidly and easily assess the potency of their cytokines.
目前测量细胞因子比活性的方法是基于对敏感细胞系生长曲线的耗时测定。在此，我们提出一种基于流式细胞术的更快替代方法，即通过测定细胞对细胞因子的剂量反应曲线来进行。通过使用世界卫生组织（WHO）的细胞因子标准品，现在可以快速测定细胞因子的比活性，因为与传统方法需要数天相比，此方法仅需几个小时就能完成，从而使实验室能够快速且轻松地评估其细胞因子的效力。
