Evavold B D, Quintans J
Department of Pathology, University of Chicago, IL 60637.
J Immunol. 1989 Sep 15;143(6):1784-9.
We have investigated the ability of T helper clones to serve as accessory cells and in the presence of mitogen activate freshly-isolated, splenic T cells. In this type of costimulatory assay, the Th cells that secrete IL-4 but not the Th cells that secrete IL-2 function as AC to induce T cell proliferation in the presence of various T cell mitogens (Con A, anti-CD3 mAb, anti-TCR mAb, and anti-Thy-1 mAb). The signal provided by the accessory Th2 cells occurred independently of MHC restriction, and the analysis of dose-response curves showed the involvement of a single stimulator cell. CD4, as well as CD8 expressing splenic T cells were induced to proliferate by the Th2 clones and mitogen, but mAb specific for CD4 or CD8 failed to affect the response. These findings indicate that cloned Th2 cells functioned as accessory cells and induced naive T cells to proliferate in the presence of mitogen.
我们研究了辅助性T细胞克隆作为辅助细胞的能力，以及在有丝分裂原存在的情况下激活新分离的脾T细胞的能力。在这种共刺激试验中，分泌IL-4的Th细胞而非分泌IL-2的Th细胞作为辅助细胞，在各种T细胞有丝分裂原（刀豆蛋白A、抗CD3单克隆抗体、抗TCR单克隆抗体和抗Thy-1单克隆抗体）存在的情况下诱导T细胞增殖。辅助性Th2细胞提供的信号独立于MHC限制，剂量反应曲线分析表明涉及单个刺激细胞。表达CD4以及CD8的脾T细胞被Th2克隆和有丝分裂原诱导增殖，但针对CD4或CD8的单克隆抗体未能影响该反应。这些发现表明，克隆的Th2细胞作为辅助细胞发挥作用，并在有丝分裂原存在的情况下诱导幼稚T细胞增殖。
