McArthur J G, Raulet D H
Department of Molecular and Cell Biology, University of California at Berkeley 94720.
J Exp Med. 1993 Nov 1;178(5):1645-53. doi: 10.1084/jem.178.5.1645.
Type 1 and type 2 cloned T helper (Th) cells are believed to require different antigen-presenting cell (APC)-derived costimuli for proliferation. In the case of Th1-cloned T cells, CD28 signaling costimulates production of autocrine interleukin 2 (IL-2). Th2 cells produce their autocrine growth factor, IL-4, without costimulation, but require APC-derived costimuli, or IL-1, to respond to IL-4. Here we demonstrate that engagement of CD28 on Th2 cells with anti-CD28 antibody or with APC-associated B7 costimulates Th2 responsiveness to IL-4 but does not affect IL-4 or IL-2 production by Th2 cells. Costimulation of Th2 cells via CD28 appears to involve the induction of IL-1 production by Th2 cells, which acts in an autocrine fashion to induce IL-4 responsiveness. These results suggest that CD28-induced costimulation plays an important role in responses mediated by both types of Th cells.
1型和2型克隆辅助性T(Th)细胞增殖时,据信需要不同的抗原呈递细胞(APC)来源的共刺激信号。对于Th1克隆T细胞,CD28信号共刺激自分泌白细胞介素2(IL-2)的产生。Th2细胞无需共刺激即可产生其自分泌生长因子IL-4,但需要APC来源的共刺激信号或IL-1才能对IL-4作出反应。在此我们证明,用抗CD28抗体或与APC相关的B7结合Th2细胞上的CD28,可共刺激Th2细胞对IL-4的反应性,但不影响Th2细胞产生IL-4或IL-2。通过CD28对Th2细胞的共刺激似乎涉及Th2细胞诱导IL-1的产生,IL-1以自分泌方式作用以诱导IL-4反应性。这些结果表明,CD28诱导的共刺激在两种类型的Th细胞介导的反应中起重要作用。