Taheri-Kafrani Asghar, Rastegari Ali Asghar, Bordbar Abdol-Kalegh
Acta Chim Slov. 2014;61(3):645-9.
Isothermal titration calorimetry (ITC) was utilized at conditions close to physiological (50 mM HEPES buffer, pH 7.4 and 160 mM NaCl) and at various temperatures of 25, 30, 35 and 40 °C to evaluate the thermodynamic parameters, enthalpy and heat capacity changes, and subsequently the unfolding process of apo-human serum tarnsferrin (apo-hTf) in the presence of cetylpyridinium chloride (CPC) as a cationic surfactant. The precise thermograms and heat capacity curves obtained and interpreted in terms of molecular events such as specific and non-specific binding and the unfolding process. The analysis of obtained enthalpograms and heat capacity changes profile showed a distinct extreme region close to [CPC]/[apo-hTf] mole ratio of 20 indicated that predominant cooperative unfolding occurs at this mole ratio via a two states mechanism.
在接近生理条件（50 mM HEPES缓冲液，pH 7.4和160 mM NaCl）以及25、30、35和40°C的不同温度下，采用等温滴定量热法（ITC）来评估热力学参数、焓变和热容变化，进而研究在作为阳离子表面活性剂的十六烷基氯化吡啶（CPC）存在下，脱铁人血清转铁蛋白（apo-hTf）的展开过程。根据特异性和非特异性结合以及展开过程等分子事件，对所获得的精确热谱图和热容曲线进行了分析和解读。对所获得的焓变图和热容变化曲线的分析表明，在[CPC]/[apo-hTf]摩尔比接近20时存在一个明显的极值区域，这表明在该摩尔比下主要通过双态机制发生协同展开。
