Puangpila Chanida, Mayadunne Erandi, El Rassi Ziad
Department of Chemistry, Oklahoma State University, Stillwater, OK, USA.
Electrophoresis. 2015 Jan;36(1):238-52. doi: 10.1002/elps.201400434. Epub 2014 Nov 24.
This review article expands on the previous one (S. Selvaraju and Z. El Rassi, Electrophoresis 2012, 33, 74-88) by reviewing pertinent literature in the period extending from early 2011 to present. As the previous review article, the present one is concerned with proteomic sample preparation (e.g., depletion of high-abundance proteins, reduction of the protein dynamic concentration range, enrichment of a particular subproteome), and the subsequent chromatographic and/or electrophoretic prefractionation prior to peptide separation and identification by LC-MS/MS. This review article, however, is distinguished from its earlier version by expanding on capturing/enriching subphosphoproteomes by immobilized metal affinity chromatography and metal oxide affinity chromatography. Seventy-seven papers published in the period extending from mid-2011 to the present have been reviewed. By no means this review article is exhaustive, given the fact that its aim is to give a concise treatment of the latest developments in the field.
这篇综述文章通过回顾2011年初至今这段时间的相关文献，对前一篇文章（S. Selvaraju和Z. El Rassi，《电泳》，2012年，第33卷，第74 - 88页）进行了扩展。与前一篇综述文章一样，本文关注蛋白质组学样品制备（例如，去除高丰度蛋白质、缩小蛋白质动态浓度范围、富集特定亚蛋白质组），以及在通过液相色谱 - 串联质谱（LC - MS/MS）进行肽段分离和鉴定之前的后续色谱和/或电泳预分级分离。然而，这篇综述文章与早期版本的不同之处在于，它扩展了通过固定金属亲和色谱和金属氧化物亲和色谱捕获/富集亚磷酸化蛋白质组的内容。本文回顾了2011年年中至今发表的77篇论文。鉴于其目的是对该领域的最新进展进行简要论述，这篇综述文章绝非详尽无遗。
