El Rassi Ziad, Puangpila Chanida
Department of Chemistry, Oklahoma State University, Stillwater, OK, USA.
Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand.
Electrophoresis. 2017 Jan;38(1):150-161. doi: 10.1002/elps.201600413. Epub 2016 Oct 31.
This review article is an update of our previous one by C. Puangpila, E. Mayadunne, and Z. El Rassi, Electrophoresis 2015, 36, 238-252. Similarly to the previous article, this review has two main topics, including (i) proteomic sample preparation (e.g., depletion of high-abundance proteins, reduction of the protein dynamic concentration range, and enrichment of a particular subproteome), and (ii) the subsequent chromatographic and/or electrophoretic prefractionation prior to protein separation and identification by LC-MS/MS. More than 70 papers published in the period extending from mid-2014 to the present have been reviewed. Although an effort was made to yield a comprehensive review article, one may safely state that this review article is by no means thorough, and the aim was to rather provide a concise description of the latest developments in the field.
这篇综述文章是我们之前由C. Puangpila、E. Mayadunne和Z. El Rassi撰写的文章（发表于《电泳》2015年第36卷，第238 - 252页）的更新版本。与之前的文章类似，本综述有两个主要主题，包括（i）蛋白质组学样品制备（例如，去除高丰度蛋白质、缩小蛋白质动态浓度范围以及富集特定的亚蛋白质组），以及（ii）在通过液相色谱 - 串联质谱（LC - MS/MS）进行蛋白质分离和鉴定之前的后续色谱和/或电泳预分离。我们对2014年年中至今发表的70多篇论文进行了综述。尽管已尽力撰写一篇全面的综述文章，但可以肯定地说，这篇综述文章绝不是详尽无遗的，其目的更在于对该领域的最新进展进行简要描述。
