[Kinetics of specific (codon-dependent) binding of aminoacyl-tRNA to the 30S ribosomal subunit under different medium conditions].

It was shown that the increase of Mg2+ ions concentration in the medium from 0,01 M to 0,03 M speeds up the formation of a codon-dependent complex between 14C-Phe-tRNA and the 30S ribosomal subparticle. Under high MgCl2 concentration (0,02 M) the increase of NH4Cl concentration also accelerates the specific binding of 14C-Phe-tRNA to the 30S subparticle. In the presence of 0,005 M MgCl2 0,5 M urea significantly decreases the rate of the specific binding. 0,5 M ethanol does not have any noticeable effect on the kinetics of the reaction.