阿片受体在体外培养的人角质形成细胞和成纤维细胞迁移及伤口愈合中的作用。

The role of opioid receptors in migration and wound recovery in vitro in cultured human keratinocytes and fibroblasts.

作者信息

Bigliardi-Qi Mei, Bigliardi Paul L

机构信息

Agency for Science Technology and Research (A*STAR), Institute of Medical Biology, 8A Biomedical Grove, Immunos Level 6 Room 610, Singapore, 138648, Singapore,

出版信息

Methods Mol Biol. 2015;1230:273-7. doi: 10.1007/978-1-4939-1708-2_23.

DOI:10.1007/978-1-4939-1708-2_23

PMID:25293334

Abstract

We have previously described significant changes in skin differentiation and the delay in wound healing from delta-opioid receptor knockout mice. In addition, we have shown that opioid receptor ligands and their receptor systems affect wound healing in vitro and the migration pattern of human skin cells, such as keratinocytes and fibroblasts (Bigliardi-Qi et al., Differentiation 74:174-185, 2006; Bigliardi et al., Exp Dermatol 18:424-430, 2009; Bigliardi et al., J Recept Signal Transduct Res 22:191-199, 2002). This observation is true for both primary keratinocytes and fibroblasts derived from foreskin or normal human skin as well as for immortalized cell lines such as HaCaT cells.

摘要

我们之前已经描述了δ阿片受体基因敲除小鼠皮肤分化的显著变化以及伤口愈合延迟的情况。此外，我们已经表明阿片受体配体及其受体系统在体外会影响伤口愈合以及人类皮肤细胞（如角质形成细胞和成纤维细胞）的迁移模式（Bigliardi-Qi等人，《分化》74:174 - 185，2006年；Bigliardi等人，《实验皮肤病学》18:424 - 430，2009年；Bigliardi等人，《受体与信号转导研究杂志》22:191 - 199，2002年）。对于源自包皮或正常人皮肤的原代角质形成细胞和成纤维细胞以及永生化细胞系（如HaCaT细胞）而言，这一观察结果都是成立的。