Department of Surgery, LKS Faculty of Medicine, The University of Hong Kong, Queen Mary Hospital, Pokfulam Road, Hong Kong SAR, China.
ChemMedChem. 2010 Mar 1;5(3):468-75. doi: 10.1002/cmdc.200900502.
With advances in nanotechnology, pure silver has been recently engineered into nanometer-sized particles (diameter <100 nm) for use in the treatment of wounds. In conjunction with other studies, we previously demonstrated that the topical application of silver nanoparticles (AgNPs) can promote wound healing through the modulation of cytokines. Nonetheless, the question as to whether AgNPs can affect various skin cell types--keratinocytes and fibroblasts--during the wound-healing process still remains. Therefore, the aim of this study was to focus on the cellular response and events of dermal contraction and epidermal re-epithelialization during wound healing under the influence of AgNPs; for this we used a full-thickness excisional wound model in mice. The wounds were treated with either AgNPs or control with silver sulfadiazine, and the proliferation and biological events of keratinocytes and fibroblasts during healing were studied. Our results confirm that AgNPs can increase the rate of wound closure. On one hand, this was achieved through the promotion of proliferation and migration of keratinocytes. On the other hand, AgNPs can drive the differentiation of fibroblasts into myofibroblasts, thereby promoting wound contraction. These findings further extend our current knowledge of AgNPs in biological and cellular events and also have significant implications for the treatment of wounds in the clinical setting.
随着纳米技术的进步，纯银最近被设计成纳米级颗粒（直径 <100nm），用于治疗伤口。结合其他研究，我们之前已经证明，银纳米颗粒（AgNPs）的局部应用可以通过调节细胞因子来促进伤口愈合。然而，AgNPs 是否会影响伤口愈合过程中的各种皮肤细胞类型（角质形成细胞和成纤维细胞）仍然是一个问题。因此，本研究的目的是关注 AgNPs 影响下的真皮收缩和表皮再上皮化的细胞反应和事件；为此，我们使用了小鼠全层切除性创面模型。创面用 AgNPs 或对照银磺胺嘧啶处理，并研究了愈合过程中角质形成细胞和成纤维细胞的增殖和生物学事件。我们的结果证实 AgNPs 可以提高创面闭合的速度。一方面，这是通过促进角质形成细胞的增殖和迁移来实现的。另一方面，AgNPs 可以促使成纤维细胞分化为肌成纤维细胞，从而促进创面收缩。这些发现进一步扩展了我们对 AgNPs 在生物学和细胞事件中的现有认识，对临床治疗创面也具有重要意义。
