Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.
Am J Hematol. 2014 Dec;89(12):1147-50. doi: 10.1002/ajh.23867.
Activated protein C resistance assays can detect factor V Leiden with high accuracy, depending on the method used. Factor Xa inhibitors such as rivaroxaban and direct thrombin inhibitors including dabigatran, argatroban, and bivalirudin can cause falsely normal results. Lupus anticoagulants can cause incorrect results in most current assays. Assays that include dilution into factor V-deficient plasma are needed to avoid interference from factor deficiencies or elevations, which can arise from a wide variety of conditions such as warfarin, liver dysfunction, or pregnancy. The pros and cons of the currently available assays are discussed.
激活蛋白 C 抵抗检测可根据所用方法准确检测到因子 V 莱顿突变。因子 Xa 抑制剂，如利伐沙班和直接凝血酶抑制剂，包括达比加群、阿加曲班和比伐卢定，可能导致假正常结果。狼疮抗凝剂可能导致大多数当前检测出现错误结果。需要进行包括稀释至因子 V 缺乏血浆的检测，以避免因各种情况（如华法林、肝功能障碍或妊娠）引起的因子缺乏或升高的干扰。目前可用的检测方法的优缺点进行了讨论。
