Li Baisheng, Tan Hailing, Wang Duochun, Ke Bixia, Chen Jingdiao, He Dongmei, Liu Meizhen, Ke Changwen, Zhang Yonghui
Center for Disease Control and Prevention of Guangdong Province, Guangzhou 511430, China; School of Public Health and Tropic Medicine, South Medical University.
Center for Disease Control and Prevention of Guangdong Province, Guangzhou 511430, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2014 Jul;35(7):825-31.
To analyze the etiologic characteristics of O1/O139 Vibrio cholerae in Guangdong province in 2009-2013.
Isolates from cholera cases and from the environment surveillance points were investigated by serological typing, antibiotic susceptibility testings, toxic genes detection and molecular typing to analyze the similarities and differences of the identified species.
Totally, 190 isolations of O1/O139 V. cholerae were obtained from cholera cases (16 strains) and environmental samples (174 strains) in Guangdong province in 2009-2013. The sero-types would include Inaba (3 isolates), Ogawa (7 isolates) and O139 (6 isolates) in all the isolates from the cholera cases. Ten strains from the ctxA positive cases were detected by PCR. Two Ogawa strains carried incomplete CTXΦ phage. Results from the antibiotic susceptibility test indicated that 5 strains were absolutely sensitive to 11 antibiotic discs in vitro, while another 3 strains were simultaneously resistant to 4 antibiotic discs. Except for 2 stains, all the O139 strains from the environment were ctxA negative, detected by PCR. Incomplete CTXΦ phage was found in the Inaba (53 isolates), Ogawa (22 isolates) and O139 (2 isolates), respectively. Results from the antibiotic susceptibility test exhibited that 25 strains were resistant simultaneously to 4 and/or more antibiotic discs, especially the Inaba strains from the seafoods(13 isolates). 2 Inaba strains from seafood were simultaneously resistant to 7 antibiotic discs. Results from PFGE molecular typing indicated that the PFGE types digested by Not I expressed significant diversity. Inaba and O139 strains from cases were gathered in the same clusters, while the Ogawa strains from cases scattered in different clusters but no significant correlation among these strains were found. Our results suggested that a distant genetic relationship might exist between these two different sources strains.
Complex and diverse as the virulence genes and genetic characteristics and with the grim situation of multi-drug resistant strains, all seemed important to strengthen the surveillance programs on the variation of strain types and antibiotics resistance of O1/O139 V. cholerae in Guangdong province.
分析2009 - 2013年广东省O1/O139群霍乱弧菌的病原学特征。
对霍乱病例及环境监测点分离株进行血清学分型、药敏试验、毒力基因检测及分子分型，分析鉴定菌株的异同。
2009 - 2013年广东省共从霍乱病例（16株）和环境样本（174株）中分离出190株O1/O139群霍乱弧菌。霍乱病例分离株的血清型包括稻叶型（3株）、小川型（7株）和O139型（6株）。对ctxA阳性病例的10株菌株进行PCR检测。2株小川型菌株携带不完整的CTXΦ噬菌体。药敏试验结果显示，5株菌株对11种抗生素纸片在体外表现为绝对敏感，而另外3株菌株同时对4种抗生素纸片耐药。除2株外，环境来源的所有O139菌株经PCR检测ctxA均为阴性。分别在稻叶型（53株）、小川型（22株）和O139型（2株）中发现不完整的CTXΦ噬菌体。药敏试验结果显示，25株菌株同时对4种及以上抗生素纸片耐药，尤其是海产品来源的稻叶型菌株（13株）。2株海产品来源的稻叶型菌株同时对7种抗生素纸片耐药。PFGE分子分型结果表明，经Not I酶切的PFGE型表现出显著的多样性。病例来源的稻叶型和O139型菌株聚集在同一簇中，而病例来源的小川型菌株分散在不同簇中，但这些菌株之间未发现显著相关性。我们的结果提示这两种不同来源的菌株之间可能存在较远的亲缘关系。
由于O1/O139群霍乱弧菌毒力基因和遗传特征复杂多样，且多重耐药菌株形势严峻，加强广东省O1/O139群霍乱弧菌菌株类型变异及抗生素耐药性的监测工作显得尤为重要。
