Overton L K, Dubins J S, de Serres F J
Center for Life Sciences and Toxicology, Research Triangle Institute, Research Triangle Park, NC 27709-2194.
Mutat Res. 1989 Oct;214(2):267-83. doi: 10.1016/0027-5107(89)90171-1.
A collection of 81 his-3 mutants of Neurospora crassa was analyzed in assays for allelic complementation and specific revertibility. In these studies, the linearity of the complementation map of the his-3 cistron (Webber, 1965) was confirmed and mutants were classified as complementing with non-polarized or polarized complementation patterns, or non-complementing. In the assays for spontaneous or induced revertibility, 89% (71/80) of the mutants reverted either spontaneously or after treatment with the chemical mutagens N-methyl-N'-nitro-N-nitrosoguanidine, ethyl methanesulfonate, 2-methoxy-6-chloro-9-(3-[ethyl-2-chloroethyl]aminopropylamino) acridine dihydrochloride, nitrous acid or hydroxylamine. The frequency of revertible mutants among the non-polarized complementing mutants was 96% (45/47), and 79% (15/19) for the polarized complementing and 79% (11/14) for the non-complementing mutants. The results of these classical genetic assays for allelic complementation and specific revertibility suggest a correlation between complementation pattern and presumptive genetic alterations at the molecular level among his-3 mutants similar to that found with ad-3B mutants induced by nitrous acid (Malling and de Serres, 1967), ethyl methanesulfonate (Malling and de Serres, 1968), or ultraviolet (Kilbey et al., 1971).
对粗糙脉孢菌的81个his-3突变体进行了等位基因互补和特异性回复突变分析。在这些研究中，证实了his-3顺反子互补图谱的线性关系（韦伯，1965年），并将突变体分类为具有非极化或极化互补模式的互补型，或非互补型。在自发或诱导回复突变分析中，89%（71/80）的突变体自发回复或在用化学诱变剂N-甲基-N'-硝基-N-亚硝基胍、甲磺酸乙酯、2-甲氧基-6-氯-9-(3-[乙基-2-氯乙基]氨基丙基氨基)吖啶二盐酸盐、亚硝酸或羟胺处理后回复。非极化互补突变体中可回复突变体的频率为96%（45/47），极化互补突变体为79%（15/19），非互补突变体为79%（11/14）。这些等位基因互补和特异性回复突变的经典遗传分析结果表明，his-3突变体的互补模式与分子水平上推测的遗传改变之间存在相关性，类似于亚硝酸（马林和德塞雷斯，1967年）、甲磺酸乙酯（马林和德塞雷斯，1968年）或紫外线（基尔比等人，1971年）诱导的ad-3B突变体所发现的相关性。
