对大量源自野外的恶性疟原虫顶膜抗原1和裂殖子表面蛋白1变体的血清反应性反映了对疟疾的季节性和终生获得性反应。
Seroreactivity to a large panel of field-derived Plasmodium falciparum apical membrane antigen 1 and merozoite surface protein 1 variants reflects seasonal and lifetime acquired responses to malaria.
作者信息
Bailey Jason A, Pablo Jozelyn, Niangaly Amadou, Travassos Mark A, Ouattara Amed, Coulibaly Drissa, Laurens Matthew B, Takala-Harrison Shannon L, Lyke Kirsten E, Skinner Jeff, Berry Andrea A, Jasinskas Algis, Nakajima-Sasaki Rie, Kouriba Bourema, Thera Mahamadou A, Felgner Philip L, Doumbo Ogobara K, Plowe Christopher V
机构信息
Howard Hughes Medical Institute/Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland; Division of Infectious Diseases, Department of Medicine, University of California, Irvine, California; Laboratory of Immunogenetics, National Institutes of Health, Bethesda, Maryland; Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, University of Sciences, Techniques and Technology, Bamako, Mali.
Howard Hughes Medical Institute/Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland; Division of Infectious Diseases, Department of Medicine, University of California, Irvine, California; Laboratory of Immunogenetics, National Institutes of Health, Bethesda, Maryland; Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, University of Sciences, Techniques and Technology, Bamako, Mali
出版信息
Am J Trop Med Hyg. 2015 Jan;92(1):9-12. doi: 10.4269/ajtmh.14-0140. Epub 2014 Oct 6.
Parasite antigen diversity poses an obstacle to developing an effective malaria vaccine. A protein microarray containing Plasmodium falciparum apical membrane antigen 1 (AMA1, n = 57) and merozoite surface protein 1 19-kD (MSP119, n = 10) variants prevalent at a malaria vaccine testing site in Bandiagara, Mali, was used to assess changes in seroreactivity caused by seasonal and lifetime exposure to malaria. Malian adults had significantly higher magnitude and breadth of seroreactivity to variants of both antigens than did Malian children. Seroreactivity increased over the course of the malaria season in children and adults, but the difference was more dramatic in children. These results help to validate diversity-covering protein microarrays as a promising tool for measuring the breadth of antibody responses to highly variant proteins, and demonstrate the potential of this new tool to help guide the development of malaria vaccines with strain-transcending efficacy.
寄生虫抗原的多样性给开发有效的疟疾疫苗带来了障碍。利用一个蛋白质微阵列来评估季节性和终生暴露于疟疾所引起的血清反应性变化，该微阵列包含在马里班迪亚拉的一个疟疾疫苗测试点流行的恶性疟原虫顶端膜抗原1（AMA1，n = 57）和裂殖子表面蛋白1 19-kD（MSP119，n = 10）变体。马里成年人对这两种抗原变体的血清反应性强度和广度显著高于马里儿童。儿童和成年人的血清反应性在疟疾季节过程中均有所增加，但儿童的差异更为显著。这些结果有助于验证覆盖多样性的蛋白质微阵列作为一种有前景的工具，用于测量针对高度可变蛋白的抗体反应广度，并证明了这种新工具在帮助指导开发具有超越菌株效力的疟疾疫苗方面的潜力。
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