Yang Lingchen, Zhao Zhiyong, Deng Yifeng, Zhou Zhenlei, Hou Jiafa
College of Veterinary Medicine, Nanjing Agricultural University, No.1 Wei-Gang, Xuanwu District, Nanjing 210095, China.
College of Veterinary Medicine, Nanjing Agricultural University, No.1 Wei-Gang, Xuanwu District, Nanjing 210095, China.
Food Chem Toxicol. 2014 Dec;74:120-30. doi: 10.1016/j.fct.2014.09.011. Epub 2014 Oct 5.
The T-2 and HT-2 toxins, the main metabolites of Fusarium poae, induce toxicity in broilers and accumulate in tissues. Consequently, during the breeding process of broilers, diets are frequently supplemented with physical adsorbents to protect birds against the toxicity induced by mycotoxins. In the present research, T-2 and HT-2 were produced in maize inoculated with F. poae. Mont, the strongest adsorbent based on in vitro adsorption ratios, was added to the contaminated diet. One-day-old chickens were randomly and equally divided into the following four groups: control diet group, Mont supplemented diet group, contaminated diet group and detoxification diet group. The experiment lasted for 42 days. Compared to the control group, the contaminated group showed significant decrease in body weight, feed intake and TP (P < 0.05), and marked increase in FCR, ALP, AST and ALT activity, T-2/HT-2 residues in the tissues and the relative expressions of apoptosis-related mRNAs (P < 0.05). Mont supplementation provided protection for the treated broilers in terms of performance, blood biochemistry, hepatic function, T-2/HT-2 residue of tissues and apoptosis. Therefore, Mont may be suitable as a detoxification agent for T-2/HT-2 in feed for broilers.
T-2毒素和HT-2毒素是禾谷镰刀菌的主要代谢产物，可在肉鸡中诱发毒性并在组织中蓄积。因此，在肉鸡养殖过程中，日粮常添加物理吸附剂以保护鸡只免受霉菌毒素诱导的毒性。在本研究中，T-2毒素和HT-2毒素由接种了禾谷镰刀菌的玉米产生。根据体外吸附率，最强的吸附剂蒙脱石被添加到受污染的日粮中。1日龄雏鸡被随机且等分为以下四组：对照日粮组、添加蒙脱石的日粮组、受污染日粮组和解毒日粮组。实验持续42天。与对照组相比，受污染组的体重、采食量和总蛋白显著降低（P<0.05），饲料转化率、碱性磷酸酶、天冬氨酸转氨酶和丙氨酸转氨酶活性、组织中T-2/HT-2残留量以及凋亡相关mRNA的相对表达显著增加（P<0.05）。添加蒙脱石在性能、血液生化、肝功能、组织中T-2/HT-2残留量和凋亡方面为受试肉鸡提供了保护。因此，蒙脱石可能适合作为肉鸡饲料中T-2/HT-2的解毒剂。
