Trent D W, Grant J A, Monath T P, Manske C L, Corina M, Fox G E
Division of Vector-Borne Viral Diseases, Centers for Disease Control, Fort Collins, Colorado 80522.
Virology. 1989 Oct;172(2):523-35. doi: 10.1016/0042-6822(89)90195-5.
Dengue 2 (DEN 2) virus strains collected from dengue hemorrhagic fever (DHF) patients and Aedes aegypti mosquitoes in Thailand, Burma, and Vietnam over a 25-year period have been analyzed by computer assisted T1-RNase-resistant oligonucleotide fingerprinting. Fifty-seven DEN 2 virus strains of the Thailand topotype were separated into four major clusters by phylogenetic analysis of 97 unique oligonucleotides identified in a common well-resolved region of the fingerprints. Similarities in the 57 fingerprints indicated that DEN 2 virus of a single, continually evolving genetic population has been involved in endemic transmission of the disease. Virus isolates from DHF cases and mosquitoes are genetically very similar, indicating that different genetic topotypes are not selectively the cause of severe DEN disease in Thailand. Microevolution of the DEN 2 virus genome from 1962-1986 was gradual with detectable changes in the pattern of oligonucleotides through time. Segregation of the DEN 2 virus fingerprints into the three decades (1960s, 1970s, and 1980s) revealed the rate of genetic change to be one consensus oligonucleotide per year. Based on average association coefficient (Sab) values between the consensus fingerprints for each decade, the similarity between the consensus fingerprints decreased by 1.4% per year. Genetic variation during each of the three decades was found to be essentially the same (0.866 +/- 0.053). Constancy in the microevolutionary rate and genetic variability suggests that a balance of genetic drift and natural selection acting on the viral population did not significantly change throughout the 25-year period.
在25年期间，从泰国、缅甸和越南的登革出血热（DHF）患者及埃及伊蚊中采集的登革2型（DEN 2）病毒株，已通过计算机辅助的T1核糖核酸酶抗性寡核苷酸指纹图谱分析。通过对指纹图谱中一个分辨率良好的共同区域鉴定出的97个独特寡核苷酸进行系统发育分析，泰国典型株的57株DEN 2病毒株被分为四个主要簇。57个指纹图谱的相似性表明，单一的、不断进化的遗传群体的DEN 2病毒参与了该疾病的地方性传播。来自DHF病例和蚊子的病毒分离株在基因上非常相似，这表明不同的基因典型株并非泰国严重登革热疾病的选择性病因。1962年至1986年期间，DEN 2病毒基因组的微进化是渐进的，随着时间的推移，寡核苷酸模式发生了可检测到的变化。将DEN 2病毒指纹图谱按三个十年（20世纪60年代、70年代和80年代）进行分类，结果显示每年有一个共有寡核苷酸发生基因变化。根据每个十年共有指纹图谱之间的平均关联系数（Sab）值，共有指纹图谱之间的相似性每年下降1.4%。发现三个十年中每个十年的遗传变异基本相同（0.866±0.053）。微进化速率和遗传变异性的恒定表明，在这25年期间，作用于病毒群体的遗传漂变和自然选择的平衡没有显著变化。
