Mohamed Azza M, Abdalla Mohga S, Rizk Maha Z, Mahdy El-Sayed M E, Farrag Abdel-Razik H, El-Sharabasy Fatma S, Aly Hanan F, Mohamed Mohamed R
Therapeutic Chemistry Department, National Research Centre, Cairo, Egypt.
Faculty of Science, Helwan University, Cairo, Egypt.
Indian J Clin Biochem. 2014 Oct;29(4):418-29. doi: 10.1007/s12291-013-0350-z. Epub 2013 Jun 14.
Anabasis articulata (Forssk) Moq. (Chenopodiaceae) is an herb, grows in Egypt, and used in folk medicine to treat diabetes, fever, and kidney infections. The protective and therapeutic effects of the ethanol extract of A. articulata aerial parts were evaluated against dimethylnitrosamine (DMN)-induced liver fibrosis, compared with the standard drug, silymarin. Hepatic hydroxyproline content, serum transforming growth factor-β1 (TGF-β1), interleukin 10 (IL-10) and fructosamine were measured as liver fibrosis markers. Hepatic malondialdehyde (MDA), nitric oxide (NO), catalase (CAT), glutathione reductase (GR) and glutathione content (GSH) were measured as oxidant/antioxidant markers. Parallel histopathological investigations were also performed. Protective and therapeutic administration of A. articulata (100 mg/kg daily for 4 weeks), markedly prevented DMN-induced loss in body and liver weights. The extract significantly inhibited the elevation of hepatic hydroxyproline, NO and MDA (P < 0.05), as well as serum fructosamine, and TGF-β1 (P < 0.05) induced by DMN while it restored IL-10 to normal level in both protective and therapeutic groups. Furthermore, A. articulata prevented the depletion in CAT, GR, and GSH levels (P ≤ 0.05). In addition, oral administration of A. articulata extract and silymarin to both protective and therapeutic groups reduced the increase in liver function enzyme activities; alanine and aspartate amintransferases, gamma-glutamyl transferase in addition to alkaline phosphatase, and caused significant increase in serum albumin concentration as compared to DMN group. These data corresponded closely with those obtained for the drug silymarin. Histopathological studies confirmed the biochemical data and revealed remarkable improvement in liver architecture. Thus, it could be concluded that, A. articulata extract exhibited in vivo hepatoprotective and therapeutic effects against DMN-induced liver injury and may act as a useful agent in controlling the progression of hepatic fibrosis through reduction of oxidative stress and improving liver function.
节状假木贼（Anabasis articulata (Forssk) Moq.，藜科）是一种草本植物，生长于埃及，在民间医学中用于治疗糖尿病、发热和肾脏感染。将节状假木贼地上部分的乙醇提取物与标准药物水飞蓟素相比较，评估其对二甲基亚硝胺（DMN）诱导的肝纤维化的保护和治疗作用。测定肝羟脯氨酸含量、血清转化生长因子-β1（TGF-β1）、白细胞介素10（IL-10）和果糖胺作为肝纤维化标志物。测定肝丙二醛（MDA）、一氧化氮（NO）、过氧化氢酶（CAT）、谷胱甘肽还原酶（GR）和谷胱甘肽含量（GSH）作为氧化/抗氧化标志物。同时进行了平行的组织病理学研究。节状假木贼（每天100mg/kg，持续4周）的保护和治疗给药显著预防了DMN诱导的体重和肝脏重量损失。该提取物显著抑制了DMN诱导的肝羟脯氨酸、NO和MDA升高（P<0.05），以及血清果糖胺和TGF-β1升高（P<0.05），同时在保护组和治疗组中使IL-10恢复到正常水平。此外，节状假木贼预防了CAT、GR和GSH水平的降低（P≤0.05）。此外，对保护组和治疗组口服节状假木贼提取物和水飞蓟素均降低了肝功能酶活性的升高；除碱性磷酸酶外，还有丙氨酸和天冬氨酸转氨酶、γ-谷氨酰转移酶，并与DMN组相比使血清白蛋白浓度显著升高。这些数据与水飞蓟素药物获得的数据密切对应。组织病理学研究证实了生化数据，并显示肝脏结构有显著改善。因此，可以得出结论，节状假木贼提取物对DMN诱导的肝损伤具有体内肝保护和治疗作用，并且可能通过降低氧化应激和改善肝功能而成为控制肝纤维化进展的有用药物。
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