Department of Digestive Diseases, The First People's Hospital of Yunnan Province, Kunming, Yunnan 650032, China.
Eur J Pharmacol. 2010 May 25;634(1-3):162-9. doi: 10.1016/j.ejphar.2010.02.022. Epub 2010 Feb 23.
Panax notoginseng saponins have recently been reported to suppress liver fibrosis. Since ginsenoside-Rg1 is the most abundant component of P.notoginseng saponins, we investigated the effect of ginsenoside-Rg1 on experimental liver fibrosis in rats. Histological analysis revealed that ginsenoside-Rg1 significantly improved the extent of liver fibrosis in rats induced by thioacetamide. Ginsenoside-Rg1 markedly suppressed the serum levels of fibrotic markers and hepatic hydroxyproline content in rats treated with thioacetamide. Ginsenoside-Rg1 also reduced the serum levels of alanine transaminase, aspartate transaminase and alkaline phosphatase. Finally, ginsenoside-Rg1 attenuated the levels of thiobarbituric acid reactive substances in livers of rats treated by thioacetamide. In cultured hepatic stellate cells, ginsenoside-Rg1 markedly inhibited cell proliferation, activation and formation of reactive oxygen species stimulated by platelet-derived growth factor-BB (PDGF-BB). Additionally, ginsenoside-Rg1 down-regulated the expression of PDGF receptor-beta by reducing the nuclear factor-kappaB activity, which was required for the gene expression. These results suggest that ginsenoside-Rg1, which exhibits its antioxidant and antifibrotic properties, may be of potential therapeutic value in protecting the liver fibrosis.
三七总皂苷最近被报道具有抑制肝纤维化的作用。由于人参皂苷-Rg1 是三七总皂苷中最丰富的成分，我们研究了人参皂苷-Rg1 对大鼠实验性肝纤维化的影响。组织学分析表明，人参皂苷-Rg1 可显著改善硫代乙酰胺诱导的大鼠肝纤维化程度。人参皂苷-Rg1 明显抑制了硫代乙酰胺处理大鼠的血清纤维化标志物和肝羟脯氨酸含量。人参皂苷-Rg1 还降低了丙氨酸转氨酶、天冬氨酸转氨酶和碱性磷酸酶的血清水平。最后，人参皂苷-Rg1 减轻了硫代乙酰胺处理大鼠肝脏中丙二醛反应物质的水平。在培养的肝星状细胞中，人参皂苷-Rg1 显著抑制血小板衍生生长因子-BB（PDGF-BB）刺激的细胞增殖、活化和活性氧的形成。此外，人参皂苷-Rg1 通过降低核因子-κB 活性下调 PDGF 受体-β的表达，核因子-κB 活性是基因表达所必需的。这些结果表明，人参皂苷-Rg1 具有抗氧化和抗纤维化特性，可能具有保护肝脏纤维化的潜在治疗价值。
