Liu Hao, Wei Wei, Sun Wu-yi, Li Xiang
Anhui Key Laboratory of Anti-inflammatory and Immunopharmacology, Anhui Engineering Technology Research Center of Anti-inflammatory and Immunodrugs, Institute of Clinical Pharmacology, Anhui Medical University, Hefei, Anhui Province, China.
J Ethnopharmacol. 2009 Apr 21;122(3):502-8. doi: 10.1016/j.jep.2009.01.035. Epub 2009 Feb 5.
Astragaloside IV is the primary pure saponin isolated from Astragalus membranaceus, one of the valuable traditional medical herbs. Antifibrotic activities of Astragalus membranaceus have been extensively proved.
To investigate the effects of astragaloside IV on hepatic stellate cells (HSCs) and hepatic fibrosis in rats induced by porcine-serum (PS).
Liver fibrosis was induced by PS injection (0.5 ml, twice a week) for 12 weeks. Astragaloside IV (2.0, 4.0 mg kg(-1)) was administered intragastrically. Liver samples were subjected to histological and immunohistochemical studies. In vitro effects of astragaloside IV on primary cultured HSCs were detected by incorporation assays.
Astragaloside IV delayed the formation of liver fibrosis and decrease the serum levels of hyaluronic acid (HA), procollagen type III (PCIII) and hydroxyproline (Hyp) content in liver. The levels of transforming growth factor-beta(1) (TGF-beta(1)) in serum and expression in liver were significantly decreased by astragaloside IV. Collagen synthesis and proliferation were significantly inhibited by astragaloside IV (1.5, 3.0, 6.0, 12.0 and 24.0 mg L(-1)) in HSCs.
The results showed that astragaloside IV displays antifibrotic effects in rats induced by PS, the mechanism by which might be associated with its inhibitory effects on collagen synthesis and proliferation in HSCs.
黄芪甲苷IV是从黄芪（一种珍贵的传统草药）中分离得到的主要纯皂苷。黄芪的抗纤维化活性已得到广泛证实。
探讨黄芪甲苷IV对猪血清（PS）诱导的大鼠肝星状细胞（HSCs）和肝纤维化的影响。
通过每周两次注射PS（0.5 ml）诱导肝纤维化12周。黄芪甲苷IV（2.0、4.0 mg kg(-1)）灌胃给药。肝脏样本进行组织学和免疫组织化学研究。通过掺入试验检测黄芪甲苷IV对原代培养的HSCs的体外作用。
黄芪甲苷IV延缓了肝纤维化的形成，降低了血清透明质酸（HA）、III型前胶原（PCIII）水平以及肝脏中羟脯氨酸（Hyp）含量。黄芪甲苷IV显著降低了血清中转化生长因子-β1（TGF-β1）水平和肝脏中的表达。黄芪甲苷IV（1.5、3.0、6.0、12.0和24.0 mg L(-1)）显著抑制了HSCs中的胶原合成和增殖。
结果表明，黄芪甲苷IV对PS诱导的大鼠具有抗纤维化作用，其机制可能与其对HSCs中胶原合成和增殖的抑制作用有关。
