Monção Nayana Bruna Nery, Costa Luciana Muratori, Arcanjo Daniel Dias Rufino, Araújo Bruno Quirino, Lustosa Maria do Carmo Gomes, Rodrigues Klinger Antônio da França, Carvalho Fernando Aécio de Amorim, Costa Amilton Paulo Raposo, Lopes Citó Antônia Maria das Graças
Department of Chemistry, Federal University of Piauí, Teresina, Piauí, Brazil.
Medicinal Plants Research Center, Federal University of Piauí, Teresina, Piauí, Brazil.
Pharmacogn Mag. 2014 Aug;10(Suppl 3):S456-62. doi: 10.4103/0973-1296.139773.
Mimosa caesalpiniifolia Benth. (Leguminosae) is widely found in the Brazilian Northeast region and markedly contributes to production of pollen and honey, being considered an important honey plant in this region.
To investigate the chemical composition of the ethanol extract of leaves from M. caesalpiniifolia by GC-MS after derivatization (silylation), as well as to evaluate the in vitro and in vivo toxicological effects and androgenic activity in rats.
The ethanol extract of leaves from Mimosa caesalpiniifolia was submitted to derivatization by silylation and analyzed by gas chromatography-mass spectrometry (GC-MS) to identification of chemical constituents. In vitro toxicological evaluation was performed by MTT assay in murine macrophages and by Artemia salina lethality assay, and the in vivo acute oral toxicity and androgenic evaluation in rats.
Totally, 32 components were detected: Phytol-TMS (11.66%), lactic acid-2TMS (9.16%), α-tocopherol-TMS (7.34%) and β-sitosterol-TMS (6.80%) were the major constituents. At the concentrations analyzed, the ethanol extract showed low cytotoxicity against brine shrimp (Artemia salina) and murine macrophages. In addition, the extract did not exhibit any toxicological effect or androgenic activity in rats.
The derivatization by silylation allowed a rapid identification of chemical compounds from the M. caesalpiniifolia leaves extract. Besides, this species presents a good safety profile as observed in toxicological studies, and possess a great potential in the production of herbal medicines or as for food consumption.
含羞草（豆科）广泛分布于巴西东北部地区，对花粉和蜂蜜的生产有显著贡献，被认为是该地区重要的蜜源植物。
通过硅烷化衍生化后的气相色谱 - 质谱联用（GC - MS）法研究含羞草叶片乙醇提取物的化学成分，并评估其对大鼠的体外和体内毒理学效应及雄激素活性。
对含羞草叶片乙醇提取物进行硅烷化衍生化处理，采用气相色谱 - 质谱联用（GC - MS）法分析以鉴定化学成分。通过MTT法在小鼠巨噬细胞中进行体外毒理学评估，并采用卤虫致死试验，以及对大鼠进行体内急性经口毒性和雄激素活性评估。
共检测到32种成分：叶绿醇 - TMS（11.66%）、乳酸 - 2TMS（9.16%）、α - 生育酚 - TMS（7.34%）和β - 谷甾醇 - TMS（6.80%）为主要成分。在所分析的浓度下，乙醇提取物对卤虫（Artemia salina）和小鼠巨噬细胞显示出低细胞毒性。此外，该提取物在大鼠中未表现出任何毒理学效应或雄激素活性。
硅烷化衍生化使得能够快速鉴定含羞草叶片提取物中的化学成分。此外，如毒理学研究所观察到的，该物种具有良好的安全性，在草药生产或食品消费方面具有巨大潜力。
