Yang Y-W, Yang Z-Y, Yan M-R, Qian Z-G, Guan K-Y
The Center for Reproducing Fine Varieties of Chinese Medicinal Plants, Yunnan College of Traditional Chinese Medicine, Kunming, Yunnan, China.
Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China.
Genet Mol Res. 2014 Oct 8;13(4):8220-4. doi: 10.4238/2014.October.8.3.
Twenty-four microsatellite markers were isolated from the genomic DNA of Amomum tsaoko Crevost et Lemaire, an important economic plant in China, using the method fast isolation by AFLP of sequences containing repeats (FIASCO). Polymorphism within each locus was assessed in 60 individuals from three populations in Yunnan Province, China, and nine of them were polymorphic. The number of alleles per polymorphic locus was 2, and the expected and observed heterozygosities ranged from 0.224 to 0.513, and from 0.050 to 0.600, respectively. Among nine microsatellite markers with polymorphism, five showed significant deviation from Hardy-Weinberg equilibrium (P < 0.01), probably due to anthropic selection and short-cloning history in cultivation. No significant linkage disequilibrium was detected between loci in our analysis. These polymorphic microsatellite markers will facilitate further studies of gene flow, population structure, identification of cultivated variety, and evaluation of germplasm resources.
利用AFLP快速分离重复序列（FIASCO）方法，从中国重要经济植物草果的基因组DNA中分离出24个微卫星标记。在中国云南省三个种群的60个个体中评估了每个位点的多态性，其中9个具有多态性。每个多态性位点的等位基因数为2，预期杂合度和观察到的杂合度分别为0.224至0.513和0.050至0.600。在9个具有多态性的微卫星标记中，有5个显示出与哈迪-温伯格平衡有显著偏差（P < 0.01），这可能是由于人为选择和栽培中的短克隆历史。在我们的分析中，未检测到位点之间存在显著的连锁不平衡。这些多态性微卫星标记将有助于进一步研究基因流动、种群结构、栽培品种鉴定和种质资源评估。
