Samprasit W, Opanasopit P, Sukma M, Kaomongkolgit R
Pharmaceutical Development of Green Innovations Group (PDGIG) Faculty of Pharmacy, Silpakorn University Nakhon Pathom, Thailand -
Minerva Stomatol. 2014 Jul-Aug;63(7-8):249-57.
This study was conducted to evaluate the antibacterial activity of Garcinia mangostana (GM) extracts on oral pathogens.
The 95% ethanol and 70% acetone extracts of the pericarp of GM was prepared and standardized by determining the amount of α-mangostin, total phenolic compounds and tannins. The antibacterial activity of GM extracts against oral pathogens was investigated by using minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC), and time kill assay. Bacterial morphology was analyzed using scanning electron microscopy (SEM).
The results indicated that the content of α-mangostin, total phenolic compounds and tannins of the both extracts were different. The 95% ethanol extract contained higher α-mangostin and total phenolic compounds. Whereas, the tannins of 70% acetone extract were significantly higher than 95% ethanol extract. The 95% ethanol extract exhibited a potent antibacterial activity with low MIC and MBC values compared to the acetone extract. The morphology of bacteria was significantly changed after treatment with extracts for 24 h. Furthermore, time kill assay revealed that bacterial cells were decreased within 2 h.
GM extracts was effective against oral bacteria pathogens. The antibacterial activity was varied by the different extraction solvents and the distinction in the contents of the compounds among extracts. These findings indicated that GM extracts showed promising antibacterial activity against oral pathogens in vitro.
本研究旨在评估山竹提取物对口腔病原菌的抗菌活性。
制备山竹果皮的95%乙醇提取物和70%丙酮提取物，并通过测定α-倒捻子素、总酚化合物和单宁的含量进行标准化。采用最低抑菌浓度（MIC）、最低杀菌浓度（MBC）和时间杀菌试验研究山竹提取物对口腔病原菌的抗菌活性。使用扫描电子显微镜（SEM）分析细菌形态。
结果表明，两种提取物中α-倒捻子素、总酚化合物和单宁的含量不同。95%乙醇提取物中α-倒捻子素和总酚化合物含量较高。而70%丙酮提取物中的单宁含量明显高于95%乙醇提取物。与丙酮提取物相比，95%乙醇提取物表现出较强的抗菌活性，MIC和MBC值较低。用提取物处理24小时后，细菌形态发生明显变化。此外，时间杀菌试验表明，细菌细胞在2小时内减少。
山竹提取物对口腔细菌病原体有效。不同提取溶剂导致抗菌活性不同，提取物中化合物含量也存在差异。这些发现表明，山竹提取物在体外对口腔病原菌显示出有前景的抗菌活性。
