Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
Pharm Biol. 2010 Feb;48(2):182-6. doi: 10.3109/13880200903062671.
The ethanol extracts of mangosteen fruit rinds prepared by several extraction methods were examined for their contents of bioactive compounds, DPPH-scavenging activity, and anti-acne producing bacteria against Propionibacterium acnes and Staphylococcus epidermidis. The dried powder of the fruit rind was extracted with 95% ethanol by maceration, percolation, Soxhlet extraction, ultrasonic extraction, and extraction using a magnetic stirrer. Soxhlet extraction promoted the maximum contents of crude extract (26.60% dry weight) and alpha-mangostin (13.51%, w/w of crude extract), and also gave the highest anti-acne activity with MIC 7.81 and 15.63 microg/mL and MBC 15.53 and 31.25 microg/mL against P. acnes and S. epidermidis, respectively. Ethanol 70% and 50% (v/v) were also compared in Soxhlet extraction. Ethanol 50% promoted the extract with maximum amounts of total phenolic compounds (26.96 g gallic acid equivalents/100 g extract) and total tannins (46.83 g tannic acid equivalents/100 g extract), and also exhibited the most effective DPPH-scavenging activity (EC(50) 12.84 microg/mL). Considering various factors involved in the process, Soxhlet extraction carried a low cost in terms of reagents and extraction time. It appears to be the recommended extraction method for mangosteen fruit rind. Ethanol 50% should be the appropriate solvent for extracting free radical-scavenging components, phenolic compounds, and tannins, while 95% ethanol is recommended for extraction of alpha-mangostin, a major anti-acne component from this plant.
几种提取方法制备的山竹果皮乙醇提取物，对其生物活性化合物含量、DPPH 清除活性以及抗痤疮产生菌（痤疮丙酸杆菌和表皮葡萄球菌）进行了研究。果皮的干燥粉末用 95%乙醇通过浸渍、渗滤、索氏提取、超声提取和搅拌器提取进行提取。索氏提取促进了粗提取物（26.60%干重）和α-倒捻子素（13.51%，粗提取物的重量比）的最大含量，并且对痤疮丙酸杆菌和表皮葡萄球菌的 MIC 分别为 7.81 和 15.63μg/mL，MBC 分别为 15.53 和 31.25μg/mL，具有最高的抗痤疮活性。还比较了索氏提取中的 70%和 50%（v/v）乙醇。50%乙醇促进了提取物中总酚类化合物（26.96g 没食子酸当量/100g 提取物）和总单宁（46.83g 鞣酸当量/100g 提取物）的最大含量，并且显示出最有效的 DPPH 清除活性（EC50为 12.84μg/mL）。考虑到该过程中涉及的各种因素，索氏提取在试剂和提取时间方面的成本较低。它似乎是山竹果皮的推荐提取方法。50%乙醇应该是提取自由基清除成分、酚类化合物和单宁的合适溶剂，而 95%乙醇则推荐用于提取该植物的主要抗痤疮成分α-倒捻子素。
