Assis Livia, Almeida Thais, Milares Luiz Paulo, dos Passos Nayara, Araújo Bruna, Bublitz Caroline, Veronez Suellen, Renno Ana Claudia Muniz
From the Department of Bioscience, Federal University of São Paulo, Santos, Sao Paulo, Brazil.
Am J Phys Med Rehabil. 2015 Aug;94(8):609-16. doi: 10.1097/PHM.0000000000000219.
The aim of this study was to evaluate the effects of an exercise training protocol and low-level laser therapy (and the association of both treatments) on musculoskeletal atrophy using an experimental model of knee osteoarthritis (OA).
Fifty male Wistar rats were randomly divided into five groups: control group, knee OA control group, OA plus exercise training group, OA plus low-level laser therapy group, and OA plus exercise training associated with low-level laser therapy group. The exercise training and the laser irradiation started 4 wks after the surgery, 3 days per week for 8 wks. The exercise was performed at a speed of 16 m/min, 3 days per week, 50 mins per day, for 8 wks. Laser irradiation was applied at two points of the left knee joint (medial and lateral), for 24 sessions.
The results showed that both trained groups (irradiated or not) presented a significant increase in the muscle cross-sectional area and a decrease in muscle fiber density compared with the knee OA control group. Moreover, both trained and laser-irradiated groups demonstrated decreased muscle-specific ring-finger protein 1 and atrogin-1 immunoexpression.
These results suggest that exercise training and low-level laser therapy were effective in preventing musculoskeletal alterations related to atrophy caused by the degenerative process induced by knee OA.
本研究旨在使用膝骨关节炎（OA）实验模型，评估运动训练方案和低强度激光疗法（以及两种治疗方法联合使用）对肌肉骨骼萎缩的影响。
将50只雄性Wistar大鼠随机分为五组：对照组、膝OA对照组、OA加运动训练组、OA加低强度激光治疗组、OA加运动训练联合低强度激光治疗组。运动训练和激光照射在手术后4周开始，每周3天，共8周。运动以16米/分钟的速度进行，每周3天，每天50分钟，共8周。在左膝关节的两个点（内侧和外侧）进行激光照射，共24次。
结果显示，与膝OA对照组相比，两个训练组（无论是否接受照射）的肌肉横截面积均显著增加，肌肉纤维密度降低。此外，训练组和激光照射组的肌肉特异性无名指蛋白1和萎缩素-1免疫表达均降低。
这些结果表明，运动训练和低强度激光疗法可有效预防与膝OA诱导的退行性过程引起的萎缩相关的肌肉骨骼改变。
