Cosson Viviane, Eschstruth Alexis, Ratet Pascal
Institut des Sciences du Végétal, Saclay Plant Sciences, CNRS, Av. de la Terrasse, 91198, Gif sur Yvette, Cedex, France.
Methods Mol Biol. 2015;1223:43-56. doi: 10.1007/978-1-4939-1695-5_4.
Legumes have been for a long time recalcitrant to efficient Agrobacterium transformation. The choice and use of model legume plants (Medicago truncatula and Lotus japonicus) for molecular studies has triggered extensive studies devoted to the development of efficient Agrobacterium-mediated transformation protocols for these two plants. In M. truncatula, transformation protocols rely on the use of highly regenerable lines obtained by recurrent in vitro culture selection. These protocols are based on Agrobacterium-mediated transformation of M. truncatula followed by somatic embryogenesis-mediated plant regeneration. We describe here the protocol developed for M. truncatula R108-1 (c3).
长期以来，豆科植物一直难以通过农杆菌进行高效转化。用于分子研究的模式豆科植物（蒺藜苜蓿和百脉根）的选择和使用引发了大量致力于为这两种植物开发高效农杆菌介导转化方案的研究。在蒺藜苜蓿中，转化方案依赖于通过反复的体外培养选择获得的高再生能力品系。这些方案基于农杆菌介导的蒺藜苜蓿转化，随后通过体细胞胚胎发生介导植株再生。我们在此描述为蒺藜苜蓿R108 - 1（c3）开发的方案。
