Pfeil T, Fischer A, Bujanowski-Weber J, Luther H, Altmeyer P, König W
Institut für Medizinische Mikrobiologie und Immunologie, Ruhr-Universität Bochum, FRG.
Immunology. 1989 Sep;68(1):37-44.
We studied the expression of the CD23 antigen as well as the release of soluble CD23 from peripheral blood lymphocytes (PBL) of atopic donors. PBL were stimulated with allergen (Dermatophagoides pteronyssinus; Der.p.), cytokines (interleukin-4, interferon-gamma; IL-4, IFN-gamma) or combinations of stimuli. CD23 levels were enhanced after the addition of allergen or IL-4 (10 U/ml) up to 10-fold compared to spontaneous expression. Soluble CD23 (sCD23) release was enhanced in a dose-dependent manner by allergen or IL-4. The combined addition of IL-4 and allergen resulted in an expression of CD23 as well as sCD23 release from PBL that was higher compared to the effects obtained with a single component. The production of Ig(E,G) increased in the presence of IL-4 (10 U/ml). This effect was enhanced after the addition of allergen. In contrast, the addition of allergen alone did not modulate IgE synthesis but revealed an enhanced IgG synthesis. Spontaneous as well as allergen- or IL-4-induced CD23 expression was significantly reduced in the presence of IFN-gamma, whereas the concomitant sCD23 release was enhanced in a dose-dependent fashion. IgE synthesis (spontaneous, IL-4 induced) was suppressed in the presence of IFN-gamma. IFN-gamma also reduced the IL-4-enhanced IgG secretion, whereas the spontaneous IgG production was not affected.
我们研究了特应性供体外周血淋巴细胞（PBL）中CD23抗原的表达以及可溶性CD23的释放情况。用变应原（屋尘螨；Der.p.）、细胞因子（白细胞介素-4、干扰素-γ；IL-4、IFN-γ）或刺激物组合刺激PBL。与自发表达相比，加入变应原或IL-4（10 U/ml）后，CD23水平提高了10倍。变应原或IL-4以剂量依赖的方式增强了可溶性CD23（sCD23）的释放。IL-4和变应原联合添加导致PBL中CD23的表达以及sCD23的释放高于单一成分的作用效果。在有IL-4（10 U/ml）存在的情况下，Ig（E，G）的产生增加。加入变应原后这种作用增强。相比之下，单独加入变应原不会调节IgE合成，但显示IgG合成增强。在IFN-γ存在的情况下，自发的以及变应原或IL-4诱导的CD23表达显著降低，而同时sCD23的释放以剂量依赖的方式增强。在IFN-γ存在的情况下，IgE合成（自发的、IL-4诱导的）受到抑制。IFN-γ还降低了IL-4增强的IgG分泌，而自发的IgG产生不受影响。
