Friedman B, Hockfield S, Black J A, Woodruff K A, Waxman S G
Department of Neurology, Yale University School of Medicine, New Haven, Connecticut 06510.
Glia. 1989;2(5):380-90. doi: 10.1002/glia.440020510.
This paper introduces "Rip" a monoclonal antibody that produces relatively complete staining of oligodendrocytes and their processes in the adult central nervous system (CNS). The distribution of Rip immunoreactivity coincides with that of myelinated axons in both the spinal cord and the cerebellum. In addition, double-immunolabeling experiments demonstrate that Rip stains processes containing myelin basic protein but does not stain processes that express glial fibrillary acidic protein. These results indicate that Rip selectively stains oligodendrocytes but not astrocytes. Moreover, individual Rip-stained oligodendrologial somata and their cytoplasmic processes were observable at both the light microscopic and electron microscopic level when the staining of myelin was reduced. This was accomplished by omitting detergents from antibody incubation steps. Rip-stained oligodendrocytes have multiple processes of varying thickness, some of which end in close proximity to myelin sheaths. These immunostained profiles, reminiscent of those observed in oligodendrocytes stained by Golgi methods, are unique to Rip and indicate that its immunoreactivity is distinct from that of existing serological markers for oligodendrocytes.
本文介绍了“Rip”，一种单克隆抗体，它能在成体中枢神经系统（CNS）中对少突胶质细胞及其突起产生相对完整的染色。Rip免疫反应性的分布与脊髓和小脑中髓鞘化轴突的分布一致。此外，双重免疫标记实验表明，Rip能对含有髓鞘碱性蛋白的突起进行染色，但不能对表达胶质纤维酸性蛋白的突起进行染色。这些结果表明，Rip能选择性地对少突胶质细胞而非星形胶质细胞进行染色。此外，当髓鞘染色减少时，在光学显微镜和电子显微镜水平均可观察到单个Rip染色的少突胶质细胞胞体及其细胞质突起。这是通过在抗体孵育步骤中省略去污剂来实现的。Rip染色的少突胶质细胞有多个厚度各异的突起，其中一些终止于靠近髓鞘的位置。这些免疫染色图像让人联想到用高尔基方法染色的少突胶质细胞所观察到的图像，是Rip所特有的，表明其免疫反应性与现有的少突胶质细胞血清学标记不同。
