Fava R A, McKanna J, Cohen S
Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee.
J Cell Physiol. 1989 Nov;141(2):284-93. doi: 10.1002/jcp.1041410209.
Lipocortin-I (p35) is a unique calcium- and phospholipid-binding protein of the lipocortin/calpactin family. Although several possibilities have been suggested, functions for the individual proteins of this family are not yet known with certainty. As an initial step in the identification of the biological function(s) of p35, we have used immunohistochemical methods to define precisely many of the cellular phenotypes that contain p35 in vivo. In all organs where p35 is found, we have observed a striking distribution of p35-positive cells. Typically it is highly enriched in a limited range of differentiated cell types while apparently totally absent from most others. Our identification of specific p35-positive cell types in vivo will now set limitations on likely possibilities for functions of this protein and thereby permit a more logical approach to the determination of its true function.
脂皮质素-I（p35）是脂皮质素/钙结合蛋白家族中一种独特的钙和磷脂结合蛋白。尽管已经提出了几种可能性，但该家族单个蛋白的功能尚未确定。作为鉴定p35生物学功能的第一步，我们使用免疫组织化学方法精确地确定了许多在体内含有p35的细胞表型。在发现p35的所有器官中，我们观察到p35阳性细胞的分布惊人。通常，它在有限范围的分化细胞类型中高度富集，而在大多数其他细胞类型中显然完全不存在。我们在体内对特定p35阳性细胞类型的鉴定现在将为该蛋白功能的可能可能性设定限制，从而允许采用更合理的方法来确定其真正功能。
