脂皮质蛋白在人支气管上皮细胞中的表达：IL-1β、TNF-α、LPS 和地塞米松的影响。
Expression of lipocortins in human bronchial epithelial cells: effects of IL-1beta , TNF-alpha, LPS and dexamethasone.
机构信息
Department of Immunology Erasmus University and Academic Hospital Dijkzigt Rotterdam The Netherlands.
出版信息
Mediators Inflamm. 1996;5(3):210-7. doi: 10.1155/S0962935196000300.
In this study, we investigated the expression of lipocortin I and II (annexin I and I in the human bronchial epithelium, both in vivo and in vitro. A clear expression of lipocortin I and II protein was found in the epithelium in sections of bronchial tissue. In cultured human bronchial epithelial cells we demonstrated the expression of lipocortin I and II mRNA and protein using Northern blotting, FACScan analysis and ELISA. No induction of lipocortin I or II mRNA or protein was observed after incubation with dexamethasone. Stimulation of bronchial epithelial cells with IL-1beta, TNF-alpha or LPS for 24 h did not affect the lipocortin I or II mRNA or protein expression, although PGE(2) and 6-keto-PGF(1alpha) production was significantly increased. This IL-1beta- and LPS-mediated increase in eicosanoids could be reduced by dexamethasone, but was not accompanied by an increase in lipocortin I or II expression. In human bronchial epithelial cells this particular glucocorticoid action is not mediated through lipocortin I or II induction.
在这项研究中，我们调查了脂皮质蛋白 I 和 II（膜联蛋白 I 和 II）在人体支气管上皮细胞中的表达，包括在体内和体外的表达。在支气管组织切片中，我们发现上皮细胞中清晰表达了脂皮质蛋白 I 和 II 蛋白。通过 Northern 印迹、FACScan 分析和 ELISA，我们在培养的人支气管上皮细胞中证实了脂皮质蛋白 I 和 II mRNA 和蛋白的表达。用地塞米松孵育后，没有观察到脂皮质蛋白 I 或 II mRNA 或蛋白的诱导。用 IL-1beta、TNF-alpha 或 LPS 刺激支气管上皮细胞 24 小时，不会影响脂皮质蛋白 I 或 II mRNA 或蛋白的表达，尽管 PGE(2) 和 6-酮-PGF(1alpha)的产生显著增加。这种 IL-1beta 和 LPS 介导的类二十烷酸的增加可以被地塞米松减少，但不会伴随着脂皮质蛋白 I 或 II 的表达增加。在人支气管上皮细胞中，这种特殊的糖皮质激素作用不是通过脂皮质蛋白 I 或 II 的诱导介导的。
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