火木层孔菌石油醚部位的体外和体内成分及抗肿瘤活性
Compositions and anti-tumor activity of Pyropolyporus fomentarius petroleum ether fraction in vitro and in vivo.
作者信息
Zhang Yanhua, Xiao Yaping, Wang Pan, Liu Quanhong
机构信息
Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, China; College of Life Sciences; Jiangsu Normal University, Xuzhou, Jiangsu, China.
Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, China.
出版信息
PLoS One. 2014 Oct 10;9(10):e109599. doi: 10.1371/journal.pone.0109599. eCollection 2014.
The chemical compositions and anti-tumor activities of the petroleum ether fraction (PE), from mushroom Pyropolyporus fomentarius, were studied. Upon gas chromatography-mass spectrometry (GC-MS) analysis, nine major constituents were identified in the fraction. In vitro, the PE showed cytotoxic activity against murine sarcoma S180 (S180) cells in a dose- and time-dependent manner, and the cytotoxic effects were associated with apoptosis. The mitochondrial membrane potential loss and the intracellular ROS generation were greatly increased in the Pyropolyporus fomentarius PE treated group, suggesting cell apoptosis, induced by the PE in S180 cells, might be mitochondria dependent and ROS mediated. Consistent with in vitro findings, the in vivo study showed that the Pyropolyporus fomentarius PE was also effective in inhibiting the tumor growth induced by S180 cells and had lower immune organ toxicity. We found that the Pyropolyporus fomentarius PE has significant anti-tumor activity and great potential in screening anti-tumor drugs.
对火木层孔菌石油醚提取物（PE）的化学成分及抗肿瘤活性进行了研究。通过气相色谱-质谱联用（GC-MS）分析，在该提取物中鉴定出9种主要成分。在体外，PE对小鼠肉瘤S180（S180）细胞具有剂量和时间依赖性的细胞毒性活性，且细胞毒性作用与细胞凋亡相关。火木层孔菌PE处理组线粒体膜电位丧失和细胞内活性氧（ROS）生成显著增加，表明PE诱导的S180细胞凋亡可能是线粒体依赖性的且由ROS介导。与体外研究结果一致，体内研究表明火木层孔菌PE在抑制S180细胞诱导的肿瘤生长方面也有效，且对免疫器官毒性较低。我们发现火木层孔菌PE具有显著的抗肿瘤活性，在筛选抗肿瘤药物方面具有巨大潜力。
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