School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan, 430074, PR China.
School of Pharmacy, Anhui University of Chinese Medicine, Anhui Key Laboratory of Modern Chinese Materia Medica, Hefei, 230012, PR China.
Phytochemistry. 2021 Mar;183:112625. doi: 10.1016/j.phytochem.2020.112625. Epub 2020 Dec 24.
The chemical constituents and their biological activities of the mushroom Pyropolyporus fomentarius were investigated in this study. Two previously undescribed pentacyclic lupane-type triterpenes, 3-formyloxybetulin and 3-formyloxybetulinic acid, two rare degraded ergosterols, pyropolincisterols A and B, along with ten known triterpenoids and four known ergosterols were isolated from the fruiting bodies of P. fomentarius. Their chemical structures were determined using a combination of spectroscopic analysis. Nine compounds exhibited certain cytotoxicities to human cancer cell lines, while polyporenic acid showed significant cytotoxicities to SMMC-7721 and A-549 with IC values less than 10 μM. Four compounds showed inhibitory activities against nitric oxide (NO) production in LPS-activated RAW264.7 macrophages with IC values of 36.3, 25.1, 21.4, and 34.2 μM, respectively. The results of this assessment suggested that the lanostane triterpenoids and ergosterols in fruiting bodies of P. fomentarius played key roles in its folk usages.
本研究对药用蘑菇硫磺菌的化学成分及其生物活性进行了研究。从硫磺菌子实体中分离得到了两种以前未描述的五环羊毛甾烷型三萜类化合物，即 3-甲酰氧基白桦脂醇和 3-甲酰氧基白桦脂酸，两种罕见的降解麦角甾醇，即硫磺菌甾醇 A 和 B，以及十种已知的三萜类化合物和四种已知的麦角甾醇。它们的化学结构是通过光谱分析结合确定的。有 9 种化合物对人癌细胞系表现出一定的细胞毒性，而多孔菌酸对 SMMC-7721 和 A-549 的细胞毒性显著，IC 值均小于 10 μM。有 4 种化合物对 LPS 激活的 RAW264.7 巨噬细胞中的一氧化氮（NO）生成具有抑制活性，IC 值分别为 36.3、25.1、21.4 和 34.2 μM。这些评估结果表明，硫磺菌子实体中的羊毛甾烷三萜类化合物和麦角甾醇在其民间用途中发挥了关键作用。
