Nam Tae-Gyu, Lee Sun Mi, Park Ji-Hae, Kim Dae-Ok, Baek Nam-in, Eom Seok Hyun
Department of Food Science and Technology, College of Life Sciences, Kyung Hee University, Yongin 446-701, Republic of Korea; Graduate School of Biotechnology and Research Institute of Life Sciences & Resources, College of Life Sciences, Kyung Hee University, Yongin 446-701, Republic of Korea.
Department of Horticultural Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 446-701, Republic of Korea.
Food Chem. 2015 Mar 1;170:97-101. doi: 10.1016/j.foodchem.2014.08.067. Epub 2014 Aug 23.
It is known that common buckwheat sprouts contain several flavonoids, including orientin, isoorientin, vitexin, isovitexin, rutin, and quercetrin, whereas tartary buckwheat sprouts contain only rutin. In this study, we evaluated flavonoids present in buckwheat sprouts and identified a previously unreported flavonoid. Simultaneous detection by HPLC was used to separate rutin and a compound that was not separated in previous studies. We used a novel HPLC elution gradient method to successfully separate rutin and the previously unidentified compound, for which we performed structural analysis. The identification of six flavonoids by HPLC was confirmed using HPLC-ESI-MS/MS analysis. The newly identified compound, M+H =611.17, was identified by NMR as the rutin epimer quercetin-3-O-robinobioside. Unlike common buckwheat sprout, tartary buckwheat sprout contained rutin as a main flavonoid, whereas other flavonoids appeared only in trace amounts or were not detected. Quercetin-3-O-robinobioside was not detected in tartary buckwheat sprout.
已知普通荞麦芽含有多种黄酮类化合物，包括荭草素、异荭草素、牡荆素、异牡荆素、芦丁和槲皮苷，而苦荞麦芽仅含有芦丁。在本研究中，我们评估了荞麦芽中存在的黄酮类化合物，并鉴定出一种以前未报道的黄酮类化合物。采用高效液相色谱（HPLC）同时检测法分离芦丁和一种在以往研究中未分离的化合物。我们使用一种新型的HPLC洗脱梯度方法成功分离了芦丁和先前未鉴定的化合物，并对其进行了结构分析。通过HPLC-ESI-MS/MS分析确认了通过HPLC鉴定的六种黄酮类化合物。新鉴定的化合物，M + H = 611.17，通过核磁共振（NMR）鉴定为芦丁差向异构体槲皮素-3-O-刺槐二糖。与普通荞麦芽不同，苦荞麦芽以芦丁作为主要黄酮类化合物，而其他黄酮类化合物仅微量出现或未被检测到。在苦荞麦芽中未检测到槲皮素-3-O-刺槐二糖。
