Institute of Environmental and Agricultural Chemistry, Università Cattolica del Sacro Cuore, via Emilia parmense 84, 29122 Piacenza, Italy.
Institute of Environmental and Agricultural Chemistry, Università Cattolica del Sacro Cuore, via Emilia parmense 84, 29122 Piacenza, Italy.
Food Chem. 2015 Mar 1;170:501-7. doi: 10.1016/j.foodchem.2014.08.034. Epub 2014 Aug 27.
The phytochemical profile of Aloe barbadensis Mill. and Aloe arborescens Mill. was investigated using colorimetric assays, triple quadrupole and time-of-flight mass spectrometry, focusing on phenolic secondary metabolites in the different leaf portions. Hydroxycinnamic acids, several characteristic anthrones and chromones, the phenolic dimer feralolide and flavonoids such as flavones and isoflavones were identified. The stable radical DPPH test and the ORAC assay were then used to determine the in vitro radical scavenging. The outer green rind was the most active, while the inner parenchyma was much less effective. The 5-methylchromones aloesin, aloeresin A and aloesone were the most active among the pure secondary metabolites tested. The results suggest that several compounds are likely to contribute to the overall radical scavenging activity, and indicate that leaf portion must be taken into account when the plant is used for its antioxidant properties.
使用比色法、三重四极杆和飞行时间质谱法研究了巴巴多斯芦荟和木立芦荟的植物化学成分谱，重点研究了不同叶片部位的酚类次生代谢物。鉴定了羟基肉桂酸、几种特征蒽酮和色酮、酚类二聚体 feralolide 和类黄酮如黄酮和异黄酮。然后使用稳定自由基 DPPH 测试和 ORAC 测定法来测定体外自由基清除能力。外层绿色外皮最活跃，而内层肉质部分则效果差得多。在测试的纯次生代谢物中，5-甲基色酮芦荟素、aloeresin A 和 aloesone 活性最高。结果表明，几种化合物可能有助于整体自由基清除活性，并表明在使用植物的抗氧化特性时必须考虑叶片部位。
