Vanderstichele H, Eechaute W, Lacroix E, Leusen I
Laboratory of Normal and Pathological Physiology, University of Ghent, Belgium.
Steroids. 1989 Jun;53(6):713-26. doi: 10.1016/0039-128x(89)90062-7.
Testicular homogenates of tamoxifen-treated rats were incubated with labeled steroid precursors (progesterone, 17 alpha-hydroxyprogesterone, dehydroepiandrosterone, androstenedione or testosterone) in order to study the effect of tamoxifen on testicular steroidogenesis. The results indicate that a 9 day treatment with a daily dose of 1 mg tamoxifen produces a reduction of the synthesis of testosterone. Inhibition of the 17 alpha-hydroxylase and C17,20-desmolase enzyme systems was observed together with an increased 20 alpha-hydroxysteroid dehydrogenase activity.
为了研究他莫昔芬对睾丸类固醇生成的影响，将经他莫昔芬处理的大鼠的睾丸匀浆与标记的类固醇前体（孕酮、17α-羟孕酮、脱氢表雄酮、雄烯二酮或睾酮）一起孵育。结果表明，每日剂量为1mg的他莫昔芬进行9天治疗会导致睾酮合成减少。观察到17α-羟化酶和C17,20-裂解酶系统受到抑制，同时20α-羟类固醇脱氢酶活性增加。
